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PMID: 201775 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Genetic relatedness of equine herpesvirus types 1 and 3.

Journal of virology ·Vol. 24 ·No. 3 ·1977-12-00 ·Pages 761-7

Allen GP, O'Callaghan DJ, Randall CC

Abstract

The genetic relatedness of two types of equine herpesviruses (EHVs), 1 (EHV-1) and 3 (EHV-3), was determined by DNA-DNA reassociation kinetics. Denatured, labeled viral DNA probe was allowed to reassociate in the presence or absence of the second unlabeled viral DNA. The initial rate of reassociation of either labeled viral DNA was increased by the presence of the heterologous viral DNA to an extent indicating only 2 to 5% homology between the two EHV genomes. Moreover, labeled RNA extracted from EHV-3-infected cells hybridized to filter-immobilized EHV-1 DNA only 2 to 3 percent as efficiently as to the homologous EHV-3 DNA. These results demonstrate that the genital (EHV-3) and nongenital (EHV-1) types of EHVs exhibit very little genetic homology.

MeSH Terms
Base Sequence DNA, Viral Genes, Viral Herpesviridae/genetics Herpesvirus 1, Equid/genetics Herpesvirus 3, Equid/genetics Nucleic Acid Hybridization RNA, Viral
Chemicals
DNA, Viral RNA, Viral
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Allen G P
O'Callaghan D J
Randall C C
References (23)
23 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.24.3.761-767.1977
Published
1977-12-00
Pages
761-7
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC515997
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.24.3.761-767.1977 PMC Full Text View journal details
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