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PMID: 20181826 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Septin 14 is involved in cortical neuronal migration via interaction with Septin 4.

Molecular biology of the cell ·Vol. 21 ·No. 8 ·2010-04-15 ·Pages 1324-34

Shinoda T, Ito H, Sudo K, Iwamoto I, Morishita R, Nagata K

Abstract

Septins are a family of conserved guanosine triphosphate/guanosine diphosphate-binding proteins implicated in a variety of cellular functions such as cell cycle control and cytokinesis. Although several members of septin family, including Septin 14 (Sept14), are abundantly expressed in nervous tissues, little is known about their physiological functions, especially in neuronal development. Here, we report that Sept14 is strongly expressed in the cortical plate of developing cerebral cortex. Knockdown experiments by using the method of in utero electroporation showed that reduction of Sept14 caused inhibition of cortical neuronal migration. Whereas cDNA encoding RNA interference-resistant Sept14 rescued the migration defect, the C-terminal deletion mutant of Sept14 did not. Biochemical analyses revealed that C-terminal coiled-coil region of Sept14 interacts with Septin 4 (Sept4). Knockdown experiments showed that Sept4 is also involved in cortical neuronal migration in vivo. In addition, knockdown of Sept14 or Sept4 inhibited leading process formation in migrating cortical neurons. These results suggest that Sept14 is involved in neuronal migration in cerebral cortex via interaction with Sept4.

MeSH Terms
Animals COS Cells Cell Division Cell Movement Cells, Cultured Cerebral Cortex/cytology Chlorocebus aethiops Cytoskeletal Proteins/chemistry,genetics,metabolism Female GTP-Binding Proteins/metabolism Gene Expression Profiling Gene Expression Regulation Gene Silencing Mice Mice, Inbred ICR Neurons/cytology,metabolism Protein Binding Protein Structure, Tertiary Pseudopodia/metabolism RNA, Small Interfering/metabolism Septins
Chemicals
Cytoskeletal Proteins RNA, Small Interfering GTP-Binding Proteins Sept14 protein, mouse Sept4 protein, mouse Septins
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Shinoda Tomoyasu
Department of Molecular Neurobiology, Aichi Human Service Center, Institute for Developmental Research, Kasugai, Aichi 480-0392, Japan.
Ito Hidenori
Sudo Kaori
Iwamoto Ikuko
Morishita Rika
Nagata Koh-ichi
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1939-4586
DOI
10.1091/mbc.e09-10-0869
Published
2010-04-15
Epub
2010-00-24
Pages
1324-34
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC2854091
Subset
IM
External Links
PubMed source DOI: 10.1091/mbc.e09-10-0869 PMC Full Text View journal details
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