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PMID: 20190762 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

A combined method for producing homogeneous glycoproteins with eukaryotic N-glycosylation.

Nature chemical biology ·Vol. 6 ·No. 4 ·2010-04-00 ·Pages 264-6

Schwarz F, Huang W, Li C, Schulz BL, Lizak C, Palumbo A, Numao S, Neri D, Aebi M, Wang LX

Abstract

We describe a new method for producing homogeneous eukaryotic N-glycoproteins. The method involves the engineering and functional transfer of the Campylobacter jejuni glycosylation machinery in Escherichia coli to express glycosylated proteins with the key GlcNAc-Asn linkage. The bacterial glycans were then trimmed and remodeled in vitro by enzymatic transglycosylation to fulfill a eukaryotic N-glycosylation. It provides a potentially general platform for producing eukaryotic N-glycoproteins.

MeSH Terms

Campylobacter jejuni/genetics,metabolism Escherichia coli/genetics,metabolism Eukaryota/chemistry,metabolism Genetic Engineering Glycoproteins/biosynthesis,chemistry Glycosylation

Chemicals

Glycoproteins

Authors & Affiliations

10 authors, click to expand affiliations / ORCID

Schwarz Flavio

Institute of Microbiology, Department of Biology, Eidgenössische Technische Hochschule (ETH) Zurich, Zurich, Switzerland.

Huang Wei

Li Cishan

Schulz Benjamin L

Lizak Christian

Palumbo Alessandro

Numao Shin

Neri Dario

Aebi Markus

Wang Lai-Xi

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Article Info

Journal

Nature chemical biology

Abbr.

Nat Chem Biol

ISSN

1552-4469

DOI

10.1038/nchembio.314

Published

2010-04-00

Epub

2010-00-28

Pages

264-6

Language

English

Region

United States

NLM ID

101231976

PMCID

PMC2842479

Subset

Grants

NIGMS NIH HHS · R01 GM080374 · United States

NIGMS NIH HHS · R01 GM080374-04 · United States

NIGMS NIH HHS · GM080374 · United States

External Links

PubMed source DOI: 10.1038/nchembio.314 PMC Full Text View journal details

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A combined method for producing homogeneous glycoproteins with eukaryotic N-glycosylation.

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