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PMID: 2019440 Published · ppublish English Journal Article

Chromosomal rearrangement in Candida stellatoidea results in a positive effect on phenotype.

Infection and immunity ·Vol. 59 ·No. 5 ·1991-05-00 ·Pages 1762-71

Wickes BL, Golin JE, Kwon-Chung KJ

Abstract

When type I Candida stellatoidea is plated onto sucrose agar at levels in excess of 10(8) cells, some isolates spontaneously form sucrose-positive colonies. These isolates do not display typical type I phenotypes but instead exhibit phenotypes intermediate between type I C. stellatoidea and C. albicans. Also, this phenotypic change only occurs in conjunction with a chromosomal rearrangement. These rearrangements have been studied in a strain naturally marked for methionine auxotrophy. Chromosome-size DNA bands separated by pulsed-field gel electrophoresis were probed with genes cloned from C. albicans. The hybridization pattern indicated that the genes on several chromosomes underwent extensive rearrangement.

MeSH Terms
Candida/genetics Chromosome Mapping Chromosomes, Fungal DNA Fingerprinting DNA, Fungal/analysis Gene Rearrangement Karyotyping Phenotype Ploidies Sucrose/metabolism
Chemicals
DNA, Fungal Sucrose
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Wickes B L
Laboratory of Clinical Investigation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892.
Golin J E
Kwon-Chung K J
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.59.5.1762-1771.1991
Published
1991-05-00
Pages
1762-71
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC257913
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.59.5.1762-1771.1991 PMC Full Text View journal details
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