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PMID: 20200280 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, N.I.H., Intramural

A critical role for the g protein-coupled receptor mFPR2 in airway inflammation and immune responses.

Journal of immunology (Baltimore, Md. : 1950) ·Vol. 184 ·No. 7 ·2010-04-01 ·Pages 3331-5

Chen K, Le Y, Liu Y, Gong W, Ying G, Huang J, Yoshimura T, Tessarollo L, Wang JM

Abstract

The formylpeptide receptor-like 1, now officially termed FPR2, in human and its mouse homolog mFPR2 mediate leukocyte migration in response to agonists associated with inflammation and immune responses. To clarify the in vivo role of the receptor, we generated mice deficient in mFPR2. mFPR2(-/-) mice showed markedly reduced severity in OVA/alum-induced allergic airway inflammation. This was associated with diminished recruitment of CD11c(+) dendritic cells into the airway mucosa and secondary lymphoid organs, as well as reduced production of Type 2 cytokines and Igs. We also found that the bronchoalveolar lavage fluid from wild type mice with airway inflammation contained mFPR2 agonist activity. This study reveals a critical role for mFPR2 in the progression of allergic airway inflammation and immune responses.

MeSH Terms
Allergens/immunology,pharmacology Alum Compounds/pharmacology Animals Bronchoalveolar Lavage Fluid/chemistry,immunology Cell Separation Chemotaxis, Leukocyte/immunology Cytokines/analysis,biosynthesis,immunology Enzyme-Linked Immunosorbent Assay Flow Cytometry Fluorescent Antibody Technique Hypersensitivity/immunology Immunohistochemistry Inflammation/immunology,pathology Lung/immunology,pathology Mice Mice, Inbred C57BL Mice, Knockout Ovalbumin/immunology,pharmacology Receptors, Formyl Peptide/immunology Respiratory Hypersensitivity/immunology,pathology Reverse Transcriptase Polymerase Chain Reaction
Chemicals
Allergens Alum Compounds Cytokines Receptors, Formyl Peptide formyl peptide receptor 2, mouse aluminum sulfate Ovalbumin
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Chen Keqiang
Laboratory of Molecular Immunoregulation, Center for Cancer Research, National Cancer Institute at Frederick, Maryland, USA.
Le Yingying
Liu Ying
Gong Wanghua
Ying Guoguang
Huang Jian
Yoshimura Teizo
Tessarollo Lino
Wang Ji Ming
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Article Info
Journal
Journal of immunology (Baltimore, Md. : 1950)
Abbr.
J Immunol
ISSN
1550-6606
DOI
10.4049/jimmunol.0903022
Published
2010-04-01
Epub
2010-00-03
Pages
3331-5
Language
English
Region
United States
NLM ID
2985117R
PMCID
PMC7330933
Subset
IM
Grants
CCR NIH HHS · HHSN261200800001C · United States
NCI NIH HHS · HHSN261200800001E · United States
Intramural NIH HHS · Z01 BC010015 · United States
Intramural NIH HHS · Z01 BC010723 · United States
External Links
PubMed source DOI: 10.4049/jimmunol.0903022 PMC Full Text View journal details
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