Construction of T-vectors, a rapid and general system for direct cloning of unmodified PCR products.

Nucleic acids research ·Vol. 19 ·No. 5 ·1991-03-11 ·Pages 1154

Marchuk D, Drumm M, Saulino A, Collins FS

Abstract

暂无摘要

MeSH Terms

Cloning, Molecular/methods Genetic Vectors Polymerase Chain Reaction

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Marchuk D

Howard Hughes Medical Institute, University of Michigan Medical Center, Ann Arbor 4810-0650.

Drumm M

Saulino A

Collins F S

References (9)

9 references, click to expand

A simple method for site-directed mutagenesis using the polymerase chain reaction.
Nucleic Acids Res. 1989 Aug 25;17(16):6545-51 PMID: 2674899
DNA mutagenesis and recombination.
Nature. 1990 Apr 19;344(6268):793-4 PMID: 2184367
PCR-induced (ligase-free) subcloning: a rapid reliable method to subclone polymerase chain reaction (PCR) products.
Nucleic Acids Res. 1990 Apr 11;18(7):1920 PMID: 2186371
Restriction endonuclease cleavage at the termini of PCR products.
Biotechniques. 1990 Sep;9(3):304, 306 PMID: 2171587
Novel non-templated nucleotide addition reactions catalyzed by procaryotic and eucaryotic DNA polymerases.
Nucleic Acids Res. 1988 Oct 25;16(20):9677-86 PMID: 2460825
Using the polymerase chain reaction to modify expression plasmids for epitope mapping.
Nucleic Acids Res. 1989 Apr 25;17(8):3319 PMID: 2471149
Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia.
Science. 1985 Dec 20;230(4732):1350-4 PMID: 2999980
Direct cloning and sequence analysis of enzymatically amplified genomic sequences.
Science. 1986 Sep 5;233(4768):1076-8 PMID: 3461561
Ligation-independent cloning of PCR products (LIC-PCR).
Nucleic Acids Res. 1990 Oct 25;18(20):6069-74 PMID: 2235490

Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

Published

1991-03-11

Pages

1154

Language

English

Region

England

NLM ID

0411011

PMCID

PMC333800

Subset

External Links

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