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PMID: 20208521 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Chemically ubiquitylated PCNA as a probe for eukaryotic translesion DNA synthesis.

Nature chemical biology ·Vol. 6 ·No. 4 ·2010-04-00 ·Pages 270-2

Chen J, Ai Y, Wang J, Haracska L, Zhuang Z

Abstract

The rapid growth in ubiquitin biology requires facile chemical approaches for protein ubiquitylation that can overcome the common problem of low yield faced by the enzymatic reaction catalyzed by ubiquitin ligases. We report a chemical approach for monoubiquitylation and SUMOylation of PCNA through disulfide exchange and intein chemistry. We used the chemically ubiquitylated and SUMOylated PCNAs in studying translesion DNA synthesis and revealed a surprising degree of flexibility of the ubiquitin modification.

MeSH Terms
Cysteine/metabolism DNA/biosynthesis,chemistry DNA-Directed DNA Polymerase/metabolism Disulfides/chemistry,metabolism Eukaryotic Cells/metabolism Inteins Molecular Probes/chemistry,metabolism Proliferating Cell Nuclear Antigen/chemistry,genetics,metabolism Protein Binding Ubiquitin/metabolism Ubiquitin-Protein Ligases/metabolism Ubiquitinated Proteins/chemistry,metabolism Ubiquitination
Chemicals
Disulfides Molecular Probes Proliferating Cell Nuclear Antigen Ubiquitin Ubiquitinated Proteins DNA Ubiquitin-Protein Ligases DNA-Directed DNA Polymerase Cysteine
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Chen Junjun
Department of Chemistry and Biochemistry, University of Delaware, Newark, Delaware, USA.
Ai Yongxing
Wang Jialiang
Haracska Lajos
Zhuang Zhihao
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Article Info
Journal
Nature chemical biology
Abbr.
Nat Chem Biol
ISSN
1552-4469
DOI
10.1038/nchembio.316
Published
2010-04-00
Epub
2010-00-07
Pages
270-2
Language
English
Region
United States
NLM ID
101231976
Subset
IM
Databases
PubChem-Substance
87464908, 87464909
External Links
PubMed source DOI: 10.1038/nchembio.316 View journal details
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