Home LiteratureArticle Details
PMID: 2022185 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Pax-3, a novel murine DNA binding protein expressed during early neurogenesis.

The EMBO journal ·Vol. 10 ·No. 5 ·1991-05-00 ·Pages 1135-47

Goulding MD, Chalepakis G, Deutsch U, Erselius JR, Gruss P

Abstract

We describe the isolation and characterization of Pax-3, a novel murine paired box gene expressed exclusively during embryogenesis. Pax-3 encodes a 479 amino acid protein with an Mr of 56 kd containing both a paired domain and a paired-type homeodomain. The Pax-3 protein is a DNA binding protein that specifically recognizes the e5 sequence present upstream of the Drosophila even-skipped gene. Pax-3 transcripts are first detected in 8.5 day mouse embryos where they are restricted to the dorsal part of the neuroepithelium and to the adjacent segmented dermomyotome. During early neurogenesis, Pax-3 expression is limited to mitotic cells in the ventricular zone of the developing spinal cord and to distinct regions in the hindbrain, midbrain and diencephalon. In 10-12 day embryos, expression of Pax-3 is also seen in neural crest cells of the developing spinal ganglia, the craniofacial mesectoderm and in limb mesenchyme of 10 and 11 day embryos.

MeSH Terms
Amino Acid Sequence Animals Base Sequence DNA-Binding Proteins/biosynthesis,genetics Drosophila/embryology,genetics Gene Expression Mesoderm/metabolism Mice Molecular Sequence Data Multigene Family Neural Crest/growth & development,metabolism,ultrastructure PAX3 Transcription Factor Paired Box Transcription Factors Transcription Factors/genetics Transcription, Genetic
Chemicals
DNA-Binding Proteins PAX3 Transcription Factor Paired Box Transcription Factors Transcription Factors Pax3 protein, mouse
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Goulding M D
Max Planck Institute for Biophysical Chemistry, Göttingen, Germany.
Chalepakis G
Deutsch U
Erselius J R
Gruss P
References (58)
58 references, click to expand
  1. Conservation of a large protein domain in the segmentation gene paired and in functionally related genes of Drosophila.
    Cell. 1986 Dec 26;47(6):1033-40 PMID: 2877747
  2. Pax2, a new murine paired-box-containing gene and its expression in the developing excretory system.
    Development. 1990 Aug;109(4):787-95 PMID: 1977574
  3. Expression of the proto-oncogene int-1 is restricted to specific neural cells in the developing mouse embryo.
    Cell. 1987 Jul 3;50(1):79-88 PMID: 3594565
  4. Transcription of H-2 and Qa genes in embryonic and adult mice.
    EMBO J. 1987 May;6(5):1265-71 PMID: 3608979
  5. An analysis of 5'-noncoding sequences from 699 vertebrate messenger RNAs.
    Nucleic Acids Res. 1987 Oct 26;15(20):8125-48 PMID: 3313277
  6. The segmentation and homeotic gene network in early Drosophila development.
    Cell. 1987 Dec 4;51(5):689-98 PMID: 2890437
  7. Expression and function of the segmentation gene fushi tarazu during Drosophila neurogenesis.
    Science. 1988 Jan 8;239(4836):170-5 PMID: 2892267
  8. Structure of two genes at the gooseberry locus related to the paired gene and their spatial expression during Drosophila embryogenesis.
    Genes Dev. 1987 Dec;1(10):1247-67 PMID: 3123319
  9. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  10. Neurogenesis in spinal cord of mouse: an autoradiographic analysis.
    Brain Res. 1978 Dec 22;159(1):1-6 PMID: 728790
  11. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.
    Biochemistry. 1979 Nov 27;18(24):5294-9 PMID: 518835
  12. The sequence 5'-AAUAAA-3'forms parts of the recognition site for polyadenylation of late SV40 mRNAs.
    Cell. 1981 Apr;24(1):251-60 PMID: 6113054
  13. Identification of early neurons in the brainstem and spinal cord. II. An autoradiographic study in the mouse.
    J Comp Neurol. 1981 Aug 1;200(2):273-88 PMID: 7287922
  14. Early development of the circumferential axonal pathway in mouse and chick spinal cord.
    J Comp Neurol. 1982 Mar 10;205(4):371-82 PMID: 7096626
  15. Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.
    Nucleic Acids Res. 1983 Mar 11;11(5):1475-89 PMID: 6828386
  16. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.
    Anal Biochem. 1983 Jul 1;132(1):6-13 PMID: 6312838
  17. Clonal analysis and cell lineages of the vertebrate central nervous system.
    Annu Rev Neurosci. 1985;8:71-102 PMID: 2580474
  18. Point mutations define a sequence flanking the AUG initiator codon that modulates translation by eukaryotic ribosomes.
    Cell. 1986 Jan 31;44(2):283-92 PMID: 3943125
  19. Spatially and temporally restricted expression of Pax2 during murine neurogenesis.
    Development. 1990 Aug;109(4):797-809 PMID: 1977575
  20. Mesodermal control of neural cell identity: floor plate induction by the notochord.
    Science. 1990 Nov 16;250(4983):985-8 PMID: 2237443
  21. The structure of the homeodomain and its functional implications.
    Trends Genet. 1990 Oct;6(10):323-9 PMID: 1980756
  22. The murine paired box gene, Pax7, is expressed specifically during the development of the nervous and muscular system.
    Mech Dev. 1990 Dec;33(1):27-37 PMID: 1982921
  23. Structure and expression pattern of the murine Hox-3.2 gene.
    Development. 1990 Oct;110(2):629-42 PMID: 1723949
  24. Pax8, a murine paired box gene expressed in the developing excretory system and thyroid gland.
    Development. 1990 Oct;110(2):643-51 PMID: 1723950
  25. Structure of the segmentation gene paired and the Drosophila PRD gene set as part of a gene network.
    Cell. 1986 Dec 5;47(5):735-46 PMID: 2877746
  26. Complementary patterns of even-skipped and fushi tarazu expression involve their differential regulation by a common set of segmentation genes in Drosophila.
    Genes Dev. 1987 Nov;1(9):981-95 PMID: 2892761
  27. Divergent homeo box proteins recognize similar DNA sequences in Drosophila.
    Nature. 1988 Apr 28;332(6167):858-61 PMID: 2895896
  28. The molecular basis for metameric pattern in the Drosophila embryo.
    Development. 1987 Sep;101(1):1-22 PMID: 2896587
  29. Pax 1, a member of a paired box homologous murine gene family, is expressed in segmented structures during development.
    Cell. 1988 May 20;53(4):617-25 PMID: 2453291
  30. Control of neuronal fate by the Drosophila segmentation gene even-skipped.
    Nature. 1988 May 26;333(6171):376-8 PMID: 3374572
  31. Expression of the homeo box-containing gene En-2 delineates a specific region of the developing mouse brain.
    Genes Dev. 1988 Mar;2(3):361-71 PMID: 2454212
  32. The molecular genetics of embryonic pattern formation in Drosophila.
    Nature. 1988 Sep 1;335(6185):25-34 PMID: 2901040
  33. The pituitary-specific transcription factor GHF-1 is a homeobox-containing protein.
    Cell. 1988 Nov 4;55(3):505-18 PMID: 2902927
  34. undulated, a mutation affecting the development of the mouse skeleton, has a point mutation in the paired box of Pax 1.
    Cell. 1988 Nov 4;55(3):531-5 PMID: 3180219
  35. daughterless, a Drosophila gene essential for both neurogenesis and sex determination, has sequence similarities to myc and the achaete-scute complex.
    Cell. 1988 Dec 23;55(6):1061-7 PMID: 3203380
  36. Activation and repression of transcription by homoeodomain-containing proteins that bind a common site.
    Nature. 1988 Dec 22-29;336(6201):744-9 PMID: 2905023
  37. Expression of homeo box genes during mouse development: a review.
    Genes Dev. 1988 Jul;2(7):773-82 PMID: 2905315
  38. The ubiquitous octamer-binding protein Oct-1 contains a POU domain with a homeo box subdomain.
    Genes Dev. 1988 Dec;2(12A):1582-99 PMID: 2905684
  39. A new DNA binding and dimerization motif in immunoglobulin enhancer binding, daughterless, MyoD, and myc proteins.
    Cell. 1989 Mar 10;56(5):777-83 PMID: 2493990
  40. Involvement of the Xenopus homeobox gene Xhox3 in pattern formation along the anterior-posterior axis.
    Cell. 1989 Apr 21;57(2):317-26 PMID: 2564813
  41. Vertebrate homeodomain proteins: families of region-specific transcription factors.
    Trends Biochem Sci. 1989 Feb;14(2):52-6 PMID: 2565051
  42. The murine and Drosophila homeobox gene complexes have common features of organization and expression.
    Cell. 1989 May 5;57(3):367-78 PMID: 2566383
  43. Maps of linkage and synteny homologies between mouse and man.
    Trends Genet. 1989 Mar;5(3):82-6 PMID: 2660365
  44. Conservation of the paired domain in metazoans and its structure in three isolated human genes.
    EMBO J. 1989 Apr;8(4):1183-90 PMID: 2501086
  45. The role of segment polarity genes during Drosophila neurogenesis.
    Genes Dev. 1989 Jun;3(6):890-904 PMID: 2501154
  46. A gene with sequence similarity to Drosophila engrailed is expressed during the development of the neural tube and vertebrae in the mouse.
    Development. 1988 Oct;104(2):305-16 PMID: 2908202
  47. Interference with function of a homeobox gene in Xenopus embryos produces malformations of the anterior spinal cord.
    Cell. 1989 Oct 6;59(1):81-93 PMID: 2477158
  48. Segmental expression of Hox-2 homoeobox-containing genes in the developing mouse hindbrain.
    Nature. 1989 Oct 5;341(6241):405-9 PMID: 2571936
  49. A single amino acid can determine the DNA binding specificity of homeodomain proteins.
    Cell. 1989 Nov 3;59(3):553-62 PMID: 2572327
  50. Isolation of two tissue-specific Drosophila paired box genes, Pox meso and Pox neuro.
    EMBO J. 1989 Nov;8(11):3447-57 PMID: 2573516
  51. A family of octamer-specific proteins present during mouse embryogenesis: evidence for germline-specific expression of an Oct factor.
    EMBO J. 1989 Sep;8(9):2543-50 PMID: 2573523
  52. Differential transcriptional activation by Oct-1 and Oct-2: interdependent activation domains induce Oct-2 phosphorylation.
    Cell. 1990 Feb 9;60(3):375-86 PMID: 2302733
  53. Effect of the notochord on proliferation and differentiation in the neural tube of the chick embryo.
    Development. 1989 Dec;107(4):793-803 PMID: 2632234
  54. Variations of cervical vertebrae after expression of a Hox-1.1 transgene in mice.
    Cell. 1990 Apr 20;61(2):301-8 PMID: 1970515
  55. The homeobox in vertebrate development.
    Curr Opin Cell Biol. 1989 Dec;1(6):1088-93 PMID: 2576857
  56. A murine even-skipped homologue, Evx 1, is expressed during early embryogenesis and neurogenesis in a biphasic manner.
    EMBO J. 1990 Jun;9(6):1839-52 PMID: 1971786
  57. Lineage, arrangement, and death of clonally related motoneurons in chick spinal cord.
    J Neurosci. 1990 Jul;10(7):2451-62 PMID: 2376781
  58. Homeo boxes in the study of development.
    Science. 1987 Jun 5;236(4806):1245-52 PMID: 2884726
Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1991-05-00
Pages
1135-47
Language
English
Region
England
NLM ID
8208664
PMCID
PMC452767
Subset
IM
Databases
GENBANK
M55398, M55399, M55400, M55401, M55402, M65224, S60919, S60920, X59082, X59358
External Links
PubMed source PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2