Home LiteratureArticle Details
PMID: 203607 Published · ppublish English Journal Article

Rapid identification of Neisseria gonorrhoeae and Neisseria meningitidis by using enzymatic profiles.

Journal of clinical microbiology ·Vol. 7 ·No. 1 ·1978-01-00 ·Pages 77-81

D'Amato RF, Eriquez LA, Tomfohrde KM, Singerman E

Abstract

The enzymatic profiles of Neisseria gonorrhoeae, N. meningitidis, and related species were determined, using a total of 48 chromogenic substrates. Enzyme classes assayed for included glycosidases, aminopeptidases, phosphoamidases, proteases, lipases, esterases, and aryl sulfatase. A final test selection of 10 substrates, based upon their differential and reproducible characteristics, allowed the separation of N. gonorrhoeae and N. meningitidis from each other and from all species tested within 4 h after primary isolation on modified Thayer-Martin medium. The need for subculturing suspect colonies from modified Thayer-Martin medium to chocolate medium with a subsequent loss of 18 to 24 h of identification is eliminated.

MeSH Terms
Aminopeptidases/metabolism Esterases/metabolism Glycoside Hydrolases/metabolism Hydrolases/metabolism Lipase/metabolism Neisseria gonorrhoeae/classification,enzymology Neisseria meningitidis/classification,enzymology Neisseriaceae/classification,enzymology Peptide Hydrolases/metabolism Phosphoric Monoester Hydrolases/metabolism
Chemicals
Hydrolases Esterases Lipase Phosphoric Monoester Hydrolases Glycoside Hydrolases Peptide Hydrolases Aminopeptidases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
D'Amato R F
Eriquez L A
Tomfohrde K M
Singerman E
References (19)
19 references, click to expand
  1. A simple fermentation medium for Neisseria gonorrhoeae.
    J Pathol Bacteriol. 1956 Apr;71(2):516-7 PMID: 13398896
  2. Phosphorolysis of maltose by enzyme preparations from Neisseria meningitidis.
    J Biol Chem. 1952 Nov;199(1):153-63 PMID: 12999827
  3. Rapid diagnosis of Enterobacteriaceae. I. Detection of bacterial glycosidases.
    Acta Pathol Microbiol Scand B. 1976 Oct;84B(5):245-51 PMID: 9774
  4. Comparison of zymogram of three lymphoblastoid cell lines with a new microtechnique.
    Biomedicine. 1976 Jun;24(3):183-90 PMID: 186133
  5. Rapid carbohydrate fermentation test for confirmation of the pathogenic Neisseria using a Ba(OH)2 indicator.
    J Clin Microbiol. 1977 Jan;5(1):15-9 PMID: 319106
  6. API ZYM: a simple rapid system for the detection of bacterial enzymes.
    J Clin Pathol. 1977 Mar;30(3):275-7 PMID: 845275
  7. Existence, localization and regulation of the biosynthesis of aminoendopeptidase in gram-negative bacteria.
    Eur J Biochem. 1976 Jun 1;65(2):517-20 PMID: 780107
  8. Carbohydrate fermentation patterns of Neisseria meningitidis determined by a microtiter method.
    Appl Microbiol. 1971 Jun;21(6):1072-4 PMID: 4327611
  9. Simplified sugar fermentation plate technique for identification of Neisseria gonorrhoeae.
    Appl Microbiol. 1974 Feb;27(2):416-7 PMID: 4207283
  10. Nutritional profiles of Neisseria gonorrhoeae, Neisseria meningitidis, and Neisseria lactamica in chemically defined media and the use of growth requirements for gonococcal typing.
    J Infect Dis. 1973 Aug;128(2):178-94 PMID: 4198720
  11. Rapid fermentation confirmation of Neisseria gonorrhoeae.
    Appl Microbiol. 1973 Apr;25(4):550-2 PMID: 4633478
  12. Modification of the rapid fermentation test for Neisseria gonorrhoeae.
    Appl Microbiol. 1974 Jun;27(6):1027-30 PMID: 4598431
  13. An improved fermentation medium for Neisseria gonorrhoeae and other Neisseria.
    Health Lab Sci. 1965 Oct;2(4):238-41 PMID: 4953822
  14. Aminopeptidase profiles of various bacteria.
    Appl Microbiol. 1967 Jul;15(4):822-5 PMID: 4963444
  15. Simple disk-plate method for the biochemical confirmation of pathogenic Neisseria.
    J Clin Microbiol. 1976 Feb;3(2):172-4 PMID: 815270
  16. Adaptation of the Minitek system for the rapid identification of Neisseria gonorrhoeae.
    J Clin Microbiol. 1976 Jan;3(1):8-13 PMID: 815269
  17. A simple carbohydrate fermentation test for identification of the pathogenic Neisseria.
    J Clin Microbiol. 1975 Jul;2(1):72-3 PMID: 818107
  18. Carbohydrate fermentation plate medium for confirmation of Neisseria species.
    J Clin Microbiol. 1975 Mar;1(3):294-7 PMID: 809474
  19. A comparative study of bacterial alanine aminohydrolases.
    Biochem Biophys Res Commun. 1967 May 5;27(3):344-9 PMID: 6035115
Article Info
Journal
Journal of clinical microbiology
Abbr.
J Clin Microbiol
ISSN
0095-1137
DOI
10.1128/jcm.7.1.77-81.1978
Published
1978-01-00
Pages
77-81
Language
English
Region
United States
NLM ID
7505564
PMCID
PMC274861
Subset
IM
External Links
PubMed source DOI: 10.1128/jcm.7.1.77-81.1978 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2