Abstract
Our analyses of three human induced pluripotent stem cell (hiPSC) and six human embryonic stem cell (hESC) lines showed marked variability in differentiation potential into specific lineages, which often hampers their differentiation into specific cell types or cell lineages of interest. Simultaneous inhibition of both Activin/Nodal and BMP pathways with small molecules, SB431542 and dorsomorphin (DM), respectively, promoted significant neural differentiation from all human pluripotent stem cell (hPSC) lines tested, regardless of their differentiation propensity. On the contrary, differentiation into other cell lineages and the number of undifferentiated cells were significantly reduced after differentiation by the dual inhibition. These results demonstrate that innate differentiation propensity of hPSCs could be overcome, at least in part, by modulation of intracellular signaling pathways, resulting in efficient generation of desirable cell types, such as neural cells.
MeSH Terms
Blotting, Western
Cell Differentiation/physiology
Cell Line
Embryonic Stem Cells/cytology
Humans
Induced Pluripotent Stem Cells/cytology
Neurons/cytology
Reverse Transcriptase Polymerase Chain Reaction
Signal Transduction/physiology
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Kim Dae-Sung
Department of Physiology, Brain Korea 21 Project for Medical Science, Yonsei University College of Medicine, [corrected] Seoul, 120-752, Korea.
Lee Jae Souk
Leem Joong Woo
Huh Yong Jun
Kim Ji Young
Kim Han-Soo
Park In-Hyun
Daley George Q
Hwang Dong-Youn
Kim Dong-Wook
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