Home LiteratureArticle Details
PMID: 20378928 Published · ppublish English Journal Article Research Support, N.I.H., Extramural

Characterization of a novel tektin member, TEKT5, in mouse sperm.

Journal of andrology ·Vol. 32 ·No. 1 ·2011-00-00 ·Pages 55-69

Cao W, Ijiri TW, Huang AP, Gerton GL

Abstract

Tektins are important components of flagella. Alterations in the expression of or mutations in mouse tektins are correlated with defective sperm motility, a cause of male infertility. Our proteomic studies of flagellar accessory structures previously identified a novel tektin, TEKT5, whose function is unknown. To understand the role of TEKT5 in mouse sperm, we characterized the expression of the mouse Tekt5 gene and the presence of TEKT5 in spermatogenic cells and spermatozoa. A complete cDNA encoding the Tekt5 transcript was assembled following reverse transcription-polymerase chain reaction (RT-PCR) and 3'-rapid amplification of cDNA ends and predicted that TEKT5 is a 62 730-dalton protein with an unusual, long C-terminus. Tekt5 mRNA was highly expressed during late stages of spermiogenesis. Among examined tissues, Tekt5 mRNA was present only in testis and brain, and quantitative RT-PCR showed that the expression level of mRNA in testis was 6.8-fold higher than that in brain. At the protein level, TEKT5 was present in sperm and was enriched in the accessory structures of flagella. Immunofluorescence confirmed that TEKT5 was localized throughout the sperm tail in flagellar accessory structures. The expression pattern suggests that TEKT5 plays an important role in flagella formation during spermiogenesis as well as being implicated in sperm motility.

MeSH Terms
Amino Acids/analysis Animals Male Mice Microtubule Proteins/biosynthesis,chemistry RNA, Messenger/metabolism Sperm Motility Sperm Tail/metabolism Spermatogenesis/physiology Spermatozoa/metabolism Testis/metabolism
Chemicals
Amino Acids Microtubule Proteins RNA, Messenger tektins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Cao Wenlei
Center for Research on Reproduction and Women's Health, University of Pennsylvania School of Medicine, 421 Curie Blvd, Philadelphia, PA 19104-6160, USA.
Ijiri Takashi W
Huang Andy P
Gerton George L
References (36)
36 references, click to expand
  1. Single amino acids determine specificity of binding of protein kinase A regulatory subunits by protein kinase A anchoring proteins.
    J Biol Chem. 1999 Oct 8;274(41):29057-62 PMID: 10506157
  2. Biochemical characterization of tektins from sperm flagellar doublet microtubules.
    J Cell Biol. 1987 Apr;104(4):1069-75 PMID: 3558479
  3. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  4. Molecular cloning and developmental expression of an mRNA encoding the 27 kDa outer dense fiber protein of rat spermatozoa.
    Mol Reprod Dev. 1994 Feb;37(2):229-40 PMID: 8179907
  5. Tektins are heterodimeric polymers in flagellar microtubules with axial periodicities matching the tubulin lattice.
    J Biol Chem. 1994 Dec 16;269(50):31800-6 PMID: 7527396
  6. Prediction and analysis of coiled-coil structures.
    Methods Enzymol. 1996;266:513-25 PMID: 8743703
  7. Optimization of real time RT-PCR methods for the analysis of gene expression in mouse eggs and preimplantation embryos.
    Mol Reprod Dev. 2005 Jul;71(3):284-9 PMID: 15806558
  8. Analysis of compositionally biased regions in sequence databases.
    Methods Enzymol. 1996;266:554-71 PMID: 8743706
  9. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
    Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350-4 PMID: 388439
  10. Absence of tektin 4 causes asthenozoospermia and subfertility in male mice.
    FASEB J. 2007 Apr;21(4):1013-25 PMID: 17244819
  11. Tektin 4 is located on outer dense fibers, not associated with axonemal tubulins of flagella in rodent spermatozoa.
    Mol Reprod Dev. 2006 Jul;73(7):929-36 PMID: 16596631
  12. The spatial and temporal expression of Tekt1, a mouse tektin C homologue, during spermatogenesis suggest that it is involved in the development of the sperm tail basal body and axoneme.
    Eur J Cell Biol. 2000 Oct;79(10):718-25 PMID: 11089920
  13. Glyceraldehyde 3-phosphate dehydrogenase-S, a sperm-specific glycolytic enzyme, is required for sperm motility and male fertility.
    Proc Natl Acad Sci U S A. 2004 Nov 23;101(47):16501-6 PMID: 15546993
  14. Testicular protein Spag5 has similarity to mitotic spindle protein Deepest and binds outer dense fiber protein Odf1.
    Mol Reprod Dev. 2001 Aug;59(4):410-6 PMID: 11468777
  15. Distribution and possible novel role of phospholipid hydroperoxide glutathione peroxidase in rat epididymal spermatozoa.
    Biol Reprod. 1997 Dec;57(6):1502-8 PMID: 9408261
  16. At least one of the protofilaments in flagellar microtubules is not composed of tubulin.
    Curr Biol. 1995 Feb 1;5(2):158-67 PMID: 7743179
  17. Dissociation of the mouse testis and characterization of isolated spermatogenic cells.
    J Histochem Cytochem. 1977 Jul;25(7):480-94 PMID: 893996
  18. The transcriptional landscape of the mammalian genome.
    Science. 2005 Sep 2;309(5740):1559-63 PMID: 16141072
  19. Molecular architecture of axonemal microtubule doublets revealed by cryo-electron tomography.
    Nature. 2006 Jul 27;442(7101):475-8 PMID: 16738547
  20. The major fibrous sheath polypeptide of mouse sperm: structural and functional similarities to the A-kinase anchoring proteins.
    Dev Biol. 1994 Sep;165(1):272-84 PMID: 8088444
  21. Mice deficient in the axonemal protein Tektin-t exhibit male infertility and immotile-cilium syndrome due to impaired inner arm dynein function.
    Mol Cell Biol. 2004 Sep;24(18):7958-64 PMID: 15340058
  22. Structural chemistry of outer dense fibers of rat sperm.
    Biol Reprod. 1980 Mar;22(2):319-32 PMID: 7378538
  23. Tektin3 encodes an evolutionarily conserved putative testicular microtubules-related protein expressed preferentially in male germ cells.
    Mol Reprod Dev. 2004 Mar;67(3):295-302 PMID: 14735490
  24. Tektin5, a new Tektin family member, is a component of the middle piece of flagella in rat spermatozoa.
    Mol Reprod Dev. 2008 Apr;75(4):650-8 PMID: 17924527
  25. Protein constituents of the mouse spermatozoon. I. An electrophoretic characterization.
    Dev Biol. 1980 Mar 15;75(2):386-404 PMID: 7372005
  26. Separation of mouse spermatogenic cells by sedimentation velocity. A morphological characterization.
    Dev Biol. 1976 Mar;49(1):119-31 PMID: 176074
  27. Tektin 3 is required for progressive sperm motility in mice.
    Mol Reprod Dev. 2009 May;76(5):453-9 PMID: 18951373
  28. A census of glutamine/asparagine-rich regions: implications for their conserved function and the prediction of novel prions.
    Proc Natl Acad Sci U S A. 2000 Oct 24;97(22):11910-5 PMID: 11050225
  29. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method.
    Methods. 2001 Dec;25(4):402-8 PMID: 11846609
  30. The importance of being proline: the interaction of proline-rich motifs in signaling proteins with their cognate domains.
    FASEB J. 2000 Feb;14(2):231-41 PMID: 10657980
  31. Adenylate kinases 1 and 2 are part of the accessory structures in the mouse sperm flagellum.
    Biol Reprod. 2006 Oct;75(4):492-500 PMID: 16790685
  32. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.
    Proc Natl Acad Sci U S A. 2002 Dec 24;99(26):16899-903 PMID: 12477932
  33. Proteomic profiling of accessory structures from the mouse sperm flagellum.
    Mol Cell Proteomics. 2006 May;5(5):801-10 PMID: 16452089
  34. Structural comparison of tektins and evidence for their determination of complex spacings in flagellar microtubules.
    J Mol Biol. 1996 Mar 29;257(2):385-97 PMID: 8609631
  35. Intrinsically disordered protein.
    J Mol Graph Model. 2001;19(1):26-59 PMID: 11381529
  36. Spag4, a novel sperm protein, binds outer dense-fiber protein Odf1 and localizes to microtubules of manchette and axoneme.
    Dev Biol. 1999 Jul 1;211(1):109-23 PMID: 10373309
Article Info
Journal
Journal of andrology
Abbr.
J Androl
ISSN
1939-4640
DOI
10.2164/jandrol.109.009456
Published
2011-00-00
Epub
2010-00-08
Pages
55-69
Language
English
Region
United States
NLM ID
8106453
PMCID
PMC3856922
Subset
IM
Grants
PHS HHS · R01-057144 · United States
NICHD NIH HHS · P01 HD006274 · United States
NICHD NIH HHS · R01 HD057144-03 · United States
NICHD NIH HHS · T32 HD007305-23 · United States
NICHD NIH HHS · R01 HD057144 · United States
NICHD NIH HHS · T32-HD007305 · United States
NICHD NIH HHS · T32 HD007305-21 · United States
NICHD NIH HHS · P01-HD006274 · United States
NICHD NIH HHS · T32 HD007305-22 · United States
NICHD NIH HHS · R01 HD057144-04 · United States
NICHD NIH HHS · P01 HD006274-35 · United States
NICHD NIH HHS · T32 HD007305 · United States
External Links
PubMed source DOI: 10.2164/jandrol.109.009456 PMC Full Text
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2