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PMID: 2038321 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Suppression of tumor formation in vivo by expression of the JE gene in malignant cells.

Molecular and cellular biology ·Vol. 11 ·No. 6 ·1991-06-00 ·Pages 3125-31

Rollins BJ, Sunday ME

Abstract

The early growth response gene JE encodes a monocyte chemoattractant, MCP-1. The JE/MCP-1 protein attracts and stimulates human monocytes and induces monocyte-mediated inhibition of tumor cell growth in vitro. Expression of human or murine JE/MCP-1 in Chinese hamster ovary (CHO) cells completely suppressed their ability to form tumors in nude mice. Coinjection of JE/MCP-1-expressing cells with nonexpressing CHO cells or with HeLa cells also prevented tumor formation. Since JE/MCP-1 expression had no discernible effect on the tranformed phenotype of these cells in vitro, the suppressive effect depends on host animal factors. These factors are likely to be components of the inflammatory response, because JE/MCP-1-expressing cells elicited a predominantly monocytic infiltrate at the site of injection. Our results suggest that JE/MCP-1 protein may be useful in cancer therapy.

Related Genes
JE
MeSH Terms
Animals Cell Division Cell Line Chemokine CCL2 Chemotactic Factors/biosynthesis,genetics,physiology Gene Expression Regulation, Neoplastic Genes, Tumor Suppressor Humans Methionine/metabolism Mice Mice, Nude Neoplasm Transplantation Neoplasms, Experimental/genetics,pathology Transfection Transplantation, Heterologous
Chemicals
Chemokine CCL2 Chemotactic Factors Methionine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Rollins B J
Division of Medicine, Dana-Farber Cancer Institute, Boston, Massachusetts 02115.
Sunday M E
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34 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.11.6.3125-3131.1991
Published
1991-06-00
Pages
3125-31
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC360158
Subset
IM
Grants
NCI NIH HHS · CA53091 · United States
NHLBI NIH HHS · HL/CA44984 · United States
External Links
PubMed source DOI: 10.1128/mcb.11.6.3125-3131.1991 PMC Full Text View journal details
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