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PMID: 20385573 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Small RNA class transition from siRNA/piRNA to miRNA during pre-implantation mouse development.

Nucleic acids research ·Vol. 38 ·No. 15 ·2010-08-00 ·Pages 5141-51

Ohnishi Y, Totoki Y, Toyoda A, Watanabe T, Yamamoto Y, Tokunaga K, Sakaki Y, Sasaki H, Hohjoh H

Abstract

Recent studies showed that small interfering RNAs (siRNAs) and Piwi-interacting RNA (piRNA) in mammalian germ cells play important roles in retrotransposon silencing and gametogenesis. However, subsequent contribution of those small RNAs to early mammalian development remains poorly understood. We investigated the expression profiles of small RNAs in mouse metaphase II oocytes, 8-16-cell stage embryos, blastocysts and the pluripotent inner cell mass (ICM) using high-throughput pyrosequencing. Here, we show that during pre-implantation development a major small RNA class changes from retrotransposon-derived small RNAs containing siRNAs and piRNAs to zygotically synthesized microRNAs (miRNAs). Some siRNAs and piRNAs are transiently upregulated and directed against specific retrotransposon classes. We also identified miRNAs expression profiles characteristic of the ICM and trophectoderm (TE) cells. Taken together, our current study reveals a major reprogramming of functional small RNAs during early mouse development from oocyte to blastocyst.

MeSH Terms
Animals Blastocyst/metabolism Blastocyst Inner Cell Mass/metabolism Cells, Cultured DEAD-box RNA Helicases/antagonists & inhibitors Embryonic Development/genetics Endoribonucleases/antagonists & inhibitors Female Mice Mice, Inbred ICR MicroRNAs/metabolism Oocytes/metabolism RNA Interference RNA, Small Interfering/genetics,metabolism Retroelements Ribonuclease III
Chemicals
MicroRNAs RNA, Small Interfering Retroelements Endoribonucleases Dicer1 protein, mouse Ribonuclease III DEAD-box RNA Helicases
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Ohnishi Yusuke
Department of Molecular Genetics, National Institute of Neuroscience, NCNP, Tokyo, Japan.
Totoki Yasushi
Toyoda Atsushi
Watanabe Toshiaki
Yamamoto Yasuhiro
Tokunaga Katsushi
Sakaki Yoshiyuki
Sasaki Hiroyuki
Hohjoh Hirohiko
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
1362-4962
DOI
10.1093/nar/gkq229
Published
2010-08-00
Epub
2010-00-12
Pages
5141-51
Language
English
Region
England
NLM ID
0411011
PMCID
PMC2926599
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/gkq229 PMC Full Text View journal details
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