Modifications of major aspects of myocardial ribonucleic acid metabolism as a response to noradrenaline. Behaviour of polyadenylate polymerase and ribonucleic acid polymerase, acetylation of histones and rate of synthesis of polyamines.

The Biochemical journal ·Vol. 168 ·No. 3 ·1977-12-15 ·Pages 333-40

Casti A, Corti A, Reali N, Mezzetti G, Orlandini G, Caldarer tcm

Abstract

Noradrenaline added to perfused rabbit heart previously perfused with labelled precursors causes, after 2.5 and 5.0 min, a general increase of specific radioactivity or RNA in subcellular fractions, but no augmentation of acetylation of F2a2 and F2a1 histone fractions and no stimulation of DNA-dependent RNA polymerase activities. Synthesis of spermidine and spermine is enhanced at 10.0 min of treatment, when there is also a fall in specific radioactivity of RNA. The cytoplasmic Mn2+-stimulated polyadenylate polymerase activity is strongly enhanced 30s to 2.5 min after injection of noradrenaline or of dibutyryl cyclic AMP. Both the cyclic nucleotide and noradrenaline have no influence in vitro on the polyadenylate polymerase reaction.

MeSH Terms

Acetylation Adenosylmethionine Decarboxylase/metabolism Animals Bucladesine/pharmacology Cytoplasm/metabolism DNA-Directed RNA Polymerases/metabolism Female Heart/drug effects Histones/metabolism In Vitro Techniques Male Manganese/metabolism Myocardium/enzymology,metabolism Norepinephrine/pharmacology Ornithine Decarboxylase/metabolism Polyamines/biosynthesis Polynucleotide Adenylyltransferase/metabolism RNA/metabolism Rabbits

Chemicals

Histones Polyamines Manganese RNA Bucladesine Polynucleotide Adenylyltransferase DNA-Directed RNA Polymerases Ornithine Decarboxylase Adenosylmethionine Decarboxylase Norepinephrine

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Casti A

Corti A

Reali N

Mezzetti G

Orlandini G

Caldarer tcm

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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj1680333

Published

1977-12-15

Pages

333-40

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1183777

Subset

External Links

PubMed source DOI: 10.1042/bj1680333 PMC Full Text View journal details

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Modifications of major aspects of myocardial ribonucleic acid metabolism as a response to noradrenaline. Behaviour of polyadenylate polymerase and ribonucleic acid polymerase, acetylation of histones and rate of synthesis of polyamines.

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