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PMID: 20439430 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

MVH in piRNA processing and gene silencing of retrotransposons.

Genes & development ·Vol. 24 ·No. 9 ·2010-05-00 ·Pages 887-92

Kuramochi-Miyagawa S, Watanabe T, Gotoh K, Takamatsu K, Chuma S, Kojima-Kita K, Shiromoto Y, Asada N, Toyoda A, Fujiyama A, Totoki Y, Shibata T, Kimura T, Nakatsuji N, Noce T, Sasaki H, Nakano T

Abstract

VASA is an evolutionarily conserved RNA helicase essential for germ cell development. The mouse PIWI family proteins MILI and MIWI2 are involved in production of Piwi-interacting RNAs (piRNAs) in fetal male germ cells through a ping-pong amplification cycle. Expression of retrotransposons is elevated in MILI- and MIWI2-deficient male germ cells due to defective de novo DNA methylation, which is presumably caused by impaired piRNA expression. Here, we report that essentially the same abnormalities are observed in MVH (mouse VASA homolog)-deficient mice. Comprehensive analysis of piRNAs in MVH-deficient fetal male germ cells showed that MVH plays crucial roles in the early phase of the ping-pong amplification cycle.

MeSH Terms
Animals Argonaute Proteins DEAD-box RNA Helicases/genetics,metabolism DNA Methylation Gene Expression Regulation Gene Silencing Genes, Intracisternal A-Particle/genetics Long Interspersed Nucleotide Elements/genetics Male Mice Mice, Knockout Protein Transport Proteins/metabolism RNA, Small Interfering/genetics,metabolism Spermatogenesis/physiology Testis/metabolism
Chemicals
Argonaute Proteins Piwil1 protein, mouse Piwil2 protein, mouse Proteins RNA, Small Interfering Ddx4 protein, mouse DEAD-box RNA Helicases
Authors & Affiliations
17 authors, click to expand affiliations / ORCID
Kuramochi-Miyagawa Satomi
Graduate School of Frontier Biosciences, Osaka University, Osaka 565-0871, Japan.
Watanabe Toshiaki
Gotoh Kengo
Takamatsu Kana
Chuma Shinichiro
Kojima-Kita Kanako
Shiromoto Yusuke
Asada Noriko
Toyoda Atsushi
Fujiyama Asao
Totoki Yasushi
Shibata Tatsuhiro
Kimura Tohru
Nakatsuji Norio
Noce Toshiaki
Sasaki Hiroyuki
Nakano Toru
References (36)
36 references, click to expand
  1. Expression and intracellular localization of mouse Vasa-homologue protein during germ cell development.
    Mech Dev. 2000 May;93(1-2):139-49 PMID: 10781947
  2. MIWI2 is essential for spermatogenesis and repression of transposons in the mouse male germline.
    Dev Cell. 2007 Apr;12(4):503-14 PMID: 17395546
  3. Ultrastructural characterization of spermatogenesis and its evolutionary conservation in the germline: germinal granules in mammals.
    Mol Cell Endocrinol. 2009 Jul 10;306(1-2):17-23 PMID: 19063939
  4. Biogenesis of small RNAs in animals.
    Nat Rev Mol Cell Biol. 2009 Feb;10(2):126-39 PMID: 19165215
  5. Lighting up mRNA localization in Drosophila oogenesis.
    Development. 2009 Aug;136(15):2493-503 PMID: 19592573
  6. A piRNA pathway primed by individual transposons is linked to de novo DNA methylation in mice.
    Mol Cell. 2008 Sep 26;31(6):785-99 PMID: 18922463
  7. Proteomic analysis of murine Piwi proteins reveals a role for arginine methylation in specifying interaction with Tudor family members.
    Genes Dev. 2009 Aug 1;23(15):1749-62 PMID: 19584108
  8. Characterization of the piRNA complex from rat testes.
    Science. 2006 Jul 21;313(5785):363-7 PMID: 16778019
  9. Discrete small RNA-generating loci as master regulators of transposon activity in Drosophila.
    Cell. 2007 Mar 23;128(6):1089-103 PMID: 17346786
  10. Argonaute proteins: mediators of RNA silencing.
    Mol Cell. 2007 Jun 8;26(5):611-23 PMID: 17560368
  11. Cytoplasmic compartmentalization of the fetal piRNA pathway in mice.
    PLoS Genet. 2009 Dec;5(12):e1000764 PMID: 20011505
  12. RNA silencing in germlines--exquisite collaboration of Argonaute proteins with small RNAs for germline survival.
    Curr Opin Cell Biol. 2009 Jun;21(3):426-34 PMID: 19303759
  13. A slicer-mediated mechanism for repeat-associated siRNA 5' end formation in Drosophila.
    Science. 2007 Mar 16;315(5818):1587-90 PMID: 17322028
  14. Specialized piRNA pathways act in germline and somatic tissues of the Drosophila ovary.
    Cell. 2009 May 1;137(3):522-35 PMID: 19395010
  15. Structural basis for RNA unwinding by the DEAD-box protein Drosophila Vasa.
    Cell. 2006 Apr 21;125(2):287-300 PMID: 16630817
  16. A novel class of small RNAs bind to MILI protein in mouse testes.
    Nature. 2006 Jul 13;442(7099):203-7 PMID: 16751777
  17. A novel class of small RNAs in mouse spermatogenic cells.
    Genes Dev. 2006 Jul 1;20(13):1709-14 PMID: 16766680
  18. Arginine methylation of Piwi proteins catalysed by dPRMT5 is required for Ago3 and Aub stability.
    Nat Cell Biol. 2009 May;11(5):652-8 PMID: 19377467
  19. Associations between PIWI proteins and TDRD1/MTR-1 are critical for integrated subcellular localization in murine male germ cells.
    Genes Cells. 2009 Oct;14(10):1155-65 PMID: 19735482
  20. Germ plasm and the differentiation of the germ cell line.
    Int Rev Cytol. 1975;43:229-80 PMID: 770367
  21. Mouse Piwi interactome identifies binding mechanism of Tdrkh Tudor domain to arginine methylated Miwi.
    Proc Natl Acad Sci U S A. 2009 Dec 1;106(48):20336-41 PMID: 19918066
  22. Loss of the Mili-interacting Tudor domain-containing protein-1 activates transposons and alters the Mili-associated small RNA profile.
    Nat Struct Mol Biol. 2009 Jun;16(6):639-46 PMID: 19465913
  23. The mouse homolog of Drosophila Vasa is required for the development of male germ cells.
    Genes Dev. 2000 Apr 1;14(7):841-53 PMID: 10766740
  24. The TDRD9-MIWI2 complex is essential for piRNA-mediated retrotransposon silencing in the mouse male germline.
    Dev Cell. 2009 Dec;17(6):775-87 PMID: 20059948
  25. miwi, a murine homolog of piwi, encodes a cytoplasmic protein essential for spermatogenesis.
    Dev Cell. 2002 Jun;2(6):819-30 PMID: 12062093
  26. Tdrd1/Mtr-1, a tudor-related gene, is essential for male germ-cell differentiation and nuage/germinal granule formation in mice.
    Proc Natl Acad Sci U S A. 2006 Oct 24;103(43):15894-9 PMID: 17038506
  27. Mili, a mammalian member of piwi family gene, is essential for spermatogenesis.
    Development. 2004 Feb;131(4):839-49 PMID: 14736746
  28. Two mouse piwi-related genes: miwi and mili.
    Mech Dev. 2001 Oct;108(1-2):121-33 PMID: 11578866
  29. The chromatoid body: a germ-cell-specific RNA-processing centre.
    Nat Rev Mol Cell Biol. 2007 Jan;8(1):85-90 PMID: 17183363
  30. A germline-specific class of small RNAs binds mammalian Piwi proteins.
    Nature. 2006 Jul 13;442(7099):199-202 PMID: 16751776
  31. A novel class of evolutionarily conserved genes defined by piwi are essential for stem cell self-renewal.
    Genes Dev. 1998 Dec 1;12(23):3715-27 PMID: 9851978
  32. piRNAs in the germ line.
    Science. 2007 Apr 20;316(5823):397 PMID: 17446387
  33. Identification and characterization of two novel classes of small RNAs in the mouse germline: retrotransposon-derived siRNAs in oocytes and germline small RNAs in testes.
    Genes Dev. 2006 Jul 1;20(13):1732-43 PMID: 16766679
  34. DNA methylation of retrotransposon genes is regulated by Piwi family members MILI and MIWI2 in murine fetal testes.
    Genes Dev. 2008 Apr 1;22(7):908-17 PMID: 18381894
  35. Vasa homolog genes in mammalian germ cell development.
    Cell Struct Funct. 2001 Jun;26(3):131-6 PMID: 11565805
  36. Mouse germ cell development during embryogenesis.
    Curr Opin Genet Dev. 2008 Aug;18(4):337-41 PMID: 18625315
Article Info
Journal
Genes & development
Abbr.
Genes Dev
ISSN
1549-5477
DOI
10.1101/gad.1902110
Published
2010-05-00
Pages
887-92
Language
English
Region
United States
NLM ID
8711660
PMCID
PMC2861188
Subset
IM
External Links
PubMed source DOI: 10.1101/gad.1902110 PMC Full Text View journal details
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