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PMID: 20439742 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Dnmt3b recruitment through E2F6 transcriptional repressor mediates germ-line gene silencing in murine somatic tissues.

Velasco G, Hubé F, Rollin J, Neuillet D, Philippe C, Bouzinba-Segard H, Galvani A, Viegas-Péquignot E, Francastel C

Abstract

Methylation of cytosine residues within the CpG dinucleotide in mammalian cells is an important mediator of gene expression, genome stability, X-chromosome inactivation, genomic imprinting, chromatin structure, and embryonic development. The majority of CpG sites in mammalian cells is methylated in a nonrandom fashion, raising the question of how DNA methylation is distributed along the genome. Here, we focused on the functions of DNA methyltransferase-3b (Dnmt3b), of which deregulated activity is linked to several human pathologies. We generated Dnmt3b hypomorphic mutant mice with reduced catalytic activity, which first revealed a deregulation of Hox genes expression, consistent with the observed homeotic transformations of the posterior axis. In addition, analysis of deregulated expression programs in Dnmt3b mutant embryos, using DNA microarrays, highlighted illegitimate activation of several germ-line genes in somatic tissues that appeared to be linked directly to their hypomethylation in mutant embryos. We provide evidence that these genes are direct targets of Dnmt3b. Moreover, the recruitment of Dnmt3b to their proximal promoter is dependant on the binding of the E2F6 transcriptional repressor, which emerges as a common hallmark in the promoters of genes found to be up-regulated as a consequence of impaired Dnmt3b activity. Therefore, our results unraveled a coordinated regulation of genes involved in meiosis, through E2F6-dependant methylation and transcriptional silencing in somatic tissues.

MeSH Terms
Animals Blotting, Western Chromatin Immunoprecipitation CpG Islands/genetics DNA (Cytosine-5-)-Methyltransferases/metabolism DNA Methylation E2F6 Transcription Factor/metabolism Gene Silencing/physiology Homeodomain Proteins/genetics Meiosis/genetics Mice Mice, Mutant Strains Oligonucleotide Array Sequence Analysis Promoter Regions, Genetic/genetics Repressor Proteins/metabolism
Chemicals
E2F6 Transcription Factor E2f6 protein, mouse Homeodomain Proteins Repressor Proteins DNA (Cytosine-5-)-Methyltransferases DNA methyltransferase 3B
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Velasco Guillaume
Centre National de la Recherche Scientifique, University Paris Diderot, 75013 Paris, France.
Hubé Florent
Rollin Jérôme
Neuillet Damien
Philippe Cathy
Bouzinba-Segard Haniaa
Galvani Angélique
Viegas-Péquignot Evani
Francastel Claire
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
1091-6490
Published
2010-05-18
Epub
2010-00-03
Pages
9281-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC2889045
Subset
IM
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