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PMID: 20448186 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Dynamic Ca2+-dependent stimulation of vesicle fusion by membrane-anchored synaptotagmin 1.

Science (New York, N.Y.) ·Vol. 328 ·No. 5979 ·2010-05-07 ·Pages 760-3

Lee HK, Yang Y, Su Z, Hyeon C, Lee TS, Lee HW, Kweon DH, Shin YK, Yoon TY

Abstract

In neurons, synaptotagmin 1 (Syt1) is thought to mediate the fusion of synaptic vesicles with the plasma membrane when presynaptic Ca2+ levels rise. However, in vitro reconstitution experiments have failed to recapitulate key characteristics of Ca2+-triggered membrane fusion. Using an in vitro single-vesicle fusion assay, we found that membrane-anchored Syt1 enhanced Ca2+ sensitivity and fusion speed. This stimulatory activity of membrane-anchored Syt1 dropped as the Ca2+ level rose beyond physiological levels. Thus, Syt1 requires the membrane anchor to stimulate vesicle fusion at physiological Ca2+ levels and may function as a dynamic presynaptic Ca2+ sensor to control the probability of neurotransmitter release.

MeSH Terms
Animals Calcium/metabolism Magnesium/metabolism Membrane Fusion Membrane Lipids/metabolism Neurotransmitter Agents/metabolism Phosphatidylinositol 4,5-Diphosphate/metabolism Rats SNARE Proteins/metabolism Synaptic Vesicles/physiology Synaptotagmin I/chemistry,metabolism
Chemicals
Membrane Lipids Neurotransmitter Agents Phosphatidylinositol 4,5-Diphosphate SNARE Proteins Synaptotagmin I Magnesium Calcium
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Lee Han-Ki
Department of Physics, KAIST, Daejeon 305-701, South Korea.
Yang Yoosoo
Su Zengliu
Hyeon Changbong
Lee Tae-Sun
Lee Hong-Won
Kweon Dae-Hyuk
Shin Yeon-Kyun
Yoon Tae-Young
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Article Info
Journal
Science (New York, N.Y.)
Abbr.
Science
ISSN
1095-9203
DOI
10.1126/science.1187722
Published
2010-05-07
Pages
760-3
Language
English
Region
United States
NLM ID
0404511
PMCID
PMC2994549
Subset
IM
Grants
NIGMS NIH HHS · R01 GM051290 · United States
NIGMS NIH HHS · R01 GM051290-16 · United States
External Links
PubMed source DOI: 10.1126/science.1187722 PMC Full Text View journal details
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