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PMID: 2045546 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Species variation in organellar location and activity of L-pipecolic acid oxidation in mammals.

Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology ·Vol. 160 ·No. 6 ·1991-00-00 ·Pages 671-6

Mihalik SJ, Rhead WJ

Abstract

The oxidation of L-pipecolic acid to alpha-aminoadipic acid was studied in eight species of mammals using an assay system more sensitive than those previously employed. After percoll-gradient fractionation, activity was localized to the mitochondrial-enriched fractions in tissues from rabbit, guinea pig, pig, dog, and sheep, with guinea pig kidney cortex showing greatest specific activity. These results contrast with the peroxisomal oxidation of L-pipecolic acid observed in macaques and man (Mihalik and Rhead 1989; Mihalik et al. 1989). Rats and mice had undetectable levels of both peroxisomal and mitochondrial L-pipecolic acid oxidation. In the rat, peroxisomal oxidation activity was not induced by feeding with either clofibrate or clofibrate and L-pipecolic acid. Thus, among mammals, both the ability to oxidize L-pipecolic acid and the organellar location of this oxidation is species dependent.

MeSH Terms
Animals Dogs Guinea Pigs Kidney Cortex/metabolism Liver/metabolism Mammals/metabolism Mice Microbodies/metabolism Mitochondria/metabolism Organelles/metabolism Oxidation-Reduction Pipecolic Acids/metabolism Rabbits Rats Sheep Species Specificity Swine
Chemicals
Pipecolic Acids pipecolic acid
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Mihalik S J
Department of Pediatrics, University of Iowa, Iowa City 52242.
Rhead W J
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31 references, click to expand
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Article Info
Journal
Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology
Abbr.
J Comp Physiol B
ISSN
0174-1578
DOI
10.1007/BF00571266
Published
1991-00-00
Pages
671-6
Language
English
Region
Germany
NLM ID
8413200
Subset
IM
Grants
NIDDK NIH HHS · DK33289 · United States
NICHD NIH HHS · HD00380 · United States
NICHD NIH HHS · HD07287 · United States
External Links
PubMed source DOI: 10.1007/BF00571266 View journal details
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