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PMID: 204665 Published · ppublish English Comparative Study Journal Article

Routine diagnosis of human rotaviruses in stools.

Journal of clinical pathology ·Vol. 31 ·No. 2 ·1978-02-00 ·Pages 175-8

Zissis G, Lambert JP, De Kegel D

Abstract

Electron microscopy, immune electron microscopy and complement fixation as methods of detecting rotavirus in the stools of young children with gastroenteritis were compared in a blind study during the winter of 1975-6. Complement fixation was the simplest to perform, was as sensitive as the other two, and allowed a quantitative measurement of viral excretion. Absorption of faecal extracts with fetal calf serum usually removed the anticomplementary activity of faecal extracts.

MeSH Terms
Child Complement Fixation Tests Diarrhea/etiology Feces/microbiology Humans RNA Viruses/isolation & purification Rotavirus/isolation & purification,ultrastructure Virus Diseases/diagnosis
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Zissis G
Lambert J P
De Kegel D
References (18)
18 references, click to expand
  1. In-vitro detection of human rotaviruses.
    Lancet. 1975 Oct 25;2(7939):821 PMID: 78193
  2. Letter: Viruses in gastroenteritis.
    Lancet. 1974 Jun 29;1(7870):1353 PMID: 4134341
  3. Letter: Virus particles in gastroenteritis.
    Lancet. 1973 Dec 29;2(7844):1497 PMID: 4129337
  4. Reoviruslike agent in stools: association with infantile diarrhea and development of serologic tests.
    Science. 1974 Sep 20;185(4156):1049-53 PMID: 4605043
  5. Letter: Virus in faecal extracts from children with gastroenteritis.
    Lancet. 1974 Jun 1;1(7866):1109 PMID: 4135267
  6. Virus particles in epithelial cells of duodenal mucosa from children with acute non-bacterial gastroenteritis.
    Lancet. 1973 Dec 8;2(7841):1281-3 PMID: 4127639
  7. Letter: Orbiviruses and gastroenteritis.
    Lancet. 1974 Sep 14;2(7881):658-9 PMID: 4137794
  8. Letter: Viral-antibody detection by a more sensitive complement-fixation reaction.
    Lancet. 1974 Apr 20;1(7860):754-5 PMID: 4132496
  9. Letter: "Rotavirus" in stored specimens of faecal extracts.
    Lancet. 1974 Nov 2;2(7888):1083 PMID: 4138251
  10. Letter: "Rotavirus" in gastroenteritis.
    Lancet. 1974 Sep 21;2(7882):726 PMID: 4143001
  11. Letter: Child-mother transmission of rotavirus.
    Lancet. 1976 Jan 10;1(7950):96 PMID: 54622
  12. Letter: Duovirus in schoolchildren with gastroenteritis.
    Lancet. 1976 Feb 7;1(7954):311 PMID: 55628
  13. Letter: Reovirus-like agent in Japanese infants with gastroenteritis.
    Lancet. 1975 Apr 19;1(7912):918-9 PMID: 47561
  14. Letter: Test for reovirus-like agent.
    Lancet. 1975 Aug 16;2(7929):322 PMID: 50527
  15. Letter: Viruses in acute summer gastroenteritis in black infants.
    Lancet. 1975 May 10;1(7915):1093-4 PMID: 48765
  16. Presence of reovirus-like particles in liquid stools.
    Acta Gastroenterol Belg. 1975 May-Jun;38(5-6):242-5 PMID: 169658
  17. Letter: Occurrence of "viral particles" in diarrhea: Houston, Texas, and Guatemala.
    N Engl J Med. 1975 Mar 20;292(12):644-5 PMID: 1113746
  18. Letter: Occurrence of "viral particles" in diarrhea: Atlanta, Georgia.
    N Engl J Med. 1975 Jun 12;292(24):1297 PMID: 1128596
Article Info
Journal
Journal of clinical pathology
Abbr.
J Clin Pathol
ISSN
0021-9746
DOI
10.1136/jcp.31.2.175
Published
1978-02-00
Pages
175-8
Language
English
Region
England
NLM ID
0376601
PMCID
PMC1145212
Subset
IM
External Links
PubMed source DOI: 10.1136/jcp.31.2.175 PMC Full Text View journal details
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