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PMID: 204930 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Triphosphate residues at the 5' ends of rRNA precursor and 5S RNA from Dictyostelium discoideum.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 75 ·No. 2 ·1978-02-00 ·Pages 740-4

Batts-Young B, Lodish HF

Abstract

We present here direct evidence for the preservation of a transcriptional initiation sequence in a eukaryotic rRNA precursor: the 5'-end group for precursor to 17S rRNA (p17S RNA) from Dictyostelium discoideum is identified as the triphosphate residue pppA-. We also show that mature 5S RNA form Dictyostelium bears a different triphosphate residue, pppG-. In contrast, we find no evidence for more than one phosphate at the 5' end of the 25S rRNA precursor (p25S RNA). These observations indicate that synthesis of the large ribosomal RNAs of Dictyostelium begins with the 5'-terminal sequence of the p17S RNA, and that 5S RNA transcription must be initiated independently, despite the close association of the 5S and rRNA coding segments.

MeSH Terms
Myxomycetes Nucleic Acid Precursors Nucleotides/analysis Phosphoric Diester Hydrolases RNA/biosynthesis RNA, Ribosomal/biosynthesis Transcription, Genetic
Chemicals
Nucleic Acid Precursors Nucleotides RNA, Ribosomal RNA Phosphoric Diester Hydrolases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Batts-Young B
Lodish H F
References (38)
38 references, click to expand
  1. Structure and function of 5S and 5.8 S RNA.
    Prog Nucleic Acid Res Mol Biol. 1976;18:45-90 PMID: 790474
  2. Organization of the ribosomal RNA genes of Dictyostelium discoideum. Mapping of the transcribed region.
    J Mol Biol. 1977 Feb 5;109(4):539-58 PMID: 557566
  3. On the distribution of 5s RNA cistrons on the genome of Saccharomyces cerevisiae.
    FEBS Lett. 1975 Mar 1;51(1):227-31 PMID: 1091508
  4. The arrangement of 18-S and 28-S ribosomal ribonucleic acids within the 40-S precursor molecule of Xenopus laevis.
    Eur J Biochem. 1976 Sep 15;68(2):471-80 PMID: 976269
  5. Measurement of 32P activity in a liquid scintillation counter without the use of scintillator.
    Anal Biochem. 1968 Jan;22(1):70-3 PMID: 5636960
  6. Conservation of the 5'-terminal nucleotide sequences of ribosomal 18-S RNA in eukaryotes. Differential evolution of large and small ribosomal RNA.
    Eur J Biochem. 1976 Apr 1;63(2):339-50 PMID: 944127
  7. Structure of a 30 S pre-ribosomal RNA of E. coli.
    FEBS Lett. 1975 Aug 1;56(1):85-91 PMID: 169158
  8. Detailed analysis of the ribosomal RNA synthesis in yeast.
    Biochim Biophys Acta. 1975 Dec 4;414(2):115-25 PMID: 1191704
  9. Studies on the amount and location of the tRNA and 5-S rRNA genes in Tetrahymena pyriformis GL.
    Eur J Biochem. 1976 Apr 1;63(2):399-407 PMID: 816653
  10. Location of the genes for 5S ribosomal RNA in Xenopus laevis.
    Chromosoma. 1973;42(2):191-203 PMID: 4726606
  11. Electrophoretic separation of viral nucleic acids on polyacrylamide gels.
    J Mol Biol. 1967 Jun 28;26(3):373-87 PMID: 5339793
  12. Precursors of ribosomal RNA in the cellular slime mold Dictyostelium discoideum. Isolation and characterization.
    J Biol Chem. 1977 Jun 10;252(11):3952-60 PMID: 558979
  13. Processing of RNA.
    Annu Rev Biochem. 1976;45:605-29 PMID: 786158
  14. Nucleotide sequence of yeast 5 S ribosomal RNA.
    FEBS Lett. 1972 Oct 1;26(1):157-60 PMID: 4636724
  15. The 5'-terminus of bacteriophage R17 RNA: pppGp.
    J Mol Biol. 1968 Jan 14;31(1):51-61 PMID: 4866114
  16. Studies of fractionated HeLa cell metaphase chromosomes. II. chromosomal distribution of sites for transfer RNA and 5 s RNA.
    J Mol Biol. 1971 Mar 28;56(3):555-63 PMID: 5573014
  17. Terminal sequences of bacteriophage RNAs.
    Nature. 1968 Nov 9;220(5167):548-52 PMID: 5686725
  18. The 5' leads to 3' polarity of the Xenopus Ribosomal RNA precursor molecule.
    Cell. 1976 Jul;8(3):449-54 PMID: 986254
  19. Sequence and conformation of 5 S RNA from Chlorella cytoplasmic ribosomes: comparison with other 5 S RNA molecules.
    J Mol Biol. 1974 Aug 5;87(2):205-25 PMID: 4427367
  20. Minor components in transfer RNA: their characterization, location, and function.
    Prog Nucleic Acid Res Mol Biol. 1972;12:49-85 PMID: 4557059
  21. Heterogneeous 5'-terminal structures occur on vesicular stomatitis virus mRNAs.
    J Biol Chem. 1975 Oct 25;250(20):8098-104 PMID: 170280
  22. Structural analysis of nucleolar precursors of ribosomal ribonucleic acid. Studies on the 5'-terminal and alkali-resistant dinucleotides of nucleolar high molecular weight ribonucleic acid.
    J Biol Chem. 1970 Apr 25;245(8):1954-61 PMID: 4314934
  23. Structure and synthesis of the ribosomal ribonucleic acid of prokaryotes.
    Bacteriol Rev. 1973 Dec;37(4):562-603 PMID: 4203396
  24. Dictyostelium 17S, 25S, and 5S rDNAs lie within a 38,000 base pair repeated unit.
    Cell. 1976 Nov;9(3):431-8 PMID: 1033038
  25. Physical linkage of the 5 S cistrons to the 18 S and 28 S ribosomal RNA cistrons in Saccharomyces cerevisiae.
    J Mol Biol. 1973 Sep 25;79(3):521-30 PMID: 4585976
  26. 5-S RNA: investigation of the different extent of phosphorylation at 5'terminus.
    Eur J Biochem. 1973 Jan 15;32(2):392-400 PMID: 4631543
  27. 5S RNA secondary structure.
    Nature. 1975 Aug 7;256(5517):505-7 PMID: 808733
  28. A reinvestigation of 5' leads to 3' polarity in 40S ribosomal RNA precursor of Xenopus laevis.
    Cell. 1976 Jul;8(3):443-8 PMID: 954098
  29. 5'-Ends of ribosomal and ribosomal precursor RNAs form Xenopus laevis.
    Eur J Biochem. 1974 Mar 15;43(1):59-67 PMID: 4600324
  30. Formylatable methionine transfer RNA from Mycoplasma: purification and comparison of partial nucleotide sequences with those of other prokaryotic initiator tRNAs.
    Nucleic Acids Res. 1975 Jan;2(1):61-78 PMID: 1093144
  31. Gene linkage by RNA-DNA hybridization. I. Unique DNA sequences homologous to 4 s RNA, 5 s RNA and ribosomal RNA.
    J Mol Biol. 1968 Jun 28;34(3):661-80 PMID: 5760467
  32. Sequence heterogeneity of 5 S RNA in Xenopus laevis.
    FEBS Lett. 1972 Sep 1;25(1):13-20 PMID: 11946710
  33. Synthesis of precursor molecules of ribosomal RNA in the cellular slime mold Dictyostelium discoideum.
    Biochim Biophys Acta. 1971 Feb 11;228(3):693-700 PMID: 5572610
  34. The 30 S ribosomal precursor RNA from Escherichia coli. A primary transcript containing 23 S, 16 S, and 5 S sequences.
    J Biol Chem. 1975 Jul 25;250(14):5647-54 PMID: 1095585
  35. Direction of chain growth in enzymic RNA synthesis.
    J Mol Biol. 1965 Sep;13(2):540-53 PMID: 5867035
  36. Localization of 5S RNA genes on Drosophila chromosomes by RNA-DNA hybridization.
    Science. 1970 Nov 6;170(3958):639-41 PMID: 5470721
  37. Specificity of initiation and synthesis of RNA from DNA templates.
    Cold Spring Harb Symp Quant Biol. 1966;31:113-22 PMID: 4866369
  38. Oligonucleotide pattern after pancreatic ribonuclease digestion and the 3' and 5' termini of 5S ribonucleic acid from HeLa cells.
    Biochemistry. 1969 Dec;8(12):4989-5005 PMID: 5365790
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.75.2.740
Published
1978-02-00
Pages
740-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC411332
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.75.2.740 PMC Full Text View journal details
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