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PMID: 20585455 Published · epublish English Journal Article

How pig sperm prepares to fertilize: stable acrosome docking to the plasma membrane.

PloS one ·Vol. 5 ·No. 6 ·2010-06-18 ·Pages e11204

Tsai PS, Garcia-Gil N, van Haeften T, Gadella BM

Abstract

Mammalian sperms are activated in the oviduct. This process, which involves extensive sperm surface remodelling, is required for fertilization and can be mimicked under in vitro fertilization conditions (IVF). Here we demonstrate that such treatments caused stable docking and priming of the acrosome membrane to the apical sperm head surface without the emergence of exocytotic membrane fusion. The interacting membranes could be isolated as bilamellar membrane structures after cell disruption. These membrane structures as well as whole capacitated sperm contained stable ternary trans-SNARE complexes that were composed of VAMP 3 and syntaxin 1B from the plasma membrane and SNAP 23 from the acrosomal membrane. This trans-SNARE complex was not observed in control sperm. We propose that this capacitation driven membrane docking and stability thereof is a preparative step prior to the multipoint membrane fusions characteristic for the acrosome reaction induced by sperm-zona binding. Thus, sperm can be considered a valuable model for studying exocytosis.

MeSH Terms
Acrosome Reaction Animals Cell Membrane/metabolism Fertilization Immunoprecipitation Male SNARE Proteins/metabolism Sperm Capacitation Spermatozoa/metabolism,physiology,ultrastructure Swine
Chemicals
SNARE Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Tsai Pei-Shiue
Department of Biochemistry and Cell Biology, Graduate School of Animal Health, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands.
Garcia-Gil Núria
van Haeften Theo
Gadella Bart M
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Article Info
Journal
PloS one
Abbr.
PLoS One
ISSN
1932-6203
DOI
10.1371/journal.pone.0011204
Published
2010-06-18
Epub
2010-00-18
Pages
e11204
Language
English
Region
United States
NLM ID
101285081
PMCID
PMC2887851
Subset
IM
External Links
PubMed source DOI: 10.1371/journal.pone.0011204 PMC Full Text View journal details
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