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PMID: 20621 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Sodium-stimulated glutamate uptake in membrane vesicles of Escherichia coli: the role of ion gradients.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 74 ·No. 8 ·1977-08-00 ·Pages 3167-70

MacDonald RE, Lanyi JK, Greene RV

Abstract

Membrane vesicles prepared from Escherichia coli B/r grown on glutamate as a sole source of carbon and energy require sodium for glutamate accumulation when energized by D-lactate oxidation. Glutamate uptake can also be driven by a prearranged sodium gradient (out to in) in the absence of an energy source or a protonmotive force. Sodium ions are exchanged rapidly in respiring vesicles and the sodium gradient may be large enough under certain conditions to drive glutamate uptake after the protonmotive force is abolished with m-chlorocarbonylcyanide phenylhydrazone. Glutamate uptake due to a prearranged sodium gradient or lactate oxidation is inhibited by monensin but not by nigericin. Transport does not occur in response to valinomycin-induced membrane potential. We interpret these results to indicate that glutamate transport is obligately coupled to sodium transport and can only occur when there is a net flux of sodium ions. This flux is driven by a chemical gradient of sodium that is created by the protonmotive force generated by respiration.

MeSH Terms
Biological Transport, Active Carbonyl Cyanide m-Chlorophenyl Hydrazone/pharmacology Cell Membrane/metabolism Escherichia coli/metabolism Glutamates/metabolism Hydrogen-Ion Concentration Kinetics Lactates/metabolism Sodium/pharmacology
Chemicals
Glutamates Lactates Carbonyl Cyanide m-Chlorophenyl Hydrazone Sodium
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
MacDonald R E
Lanyi J K
Greene R V
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26 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.74.8.3167
Published
1977-08-00
Pages
3167-70
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC431480
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.74.8.3167 PMC Full Text View journal details
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