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PMID: 20629700 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

O-antigen diversity and lateral transfer of the wbe region among Vibrio splendidus isolates.

Environmental microbiology ·Vol. 12 ·No. 11 ·2010-11-00 ·Pages 2977-87

Wildschutte H, Preheim SP, Hernandez Y, Polz MF

Abstract

The O-antigen is a highly diverse structure expressed on the outer surface of Gram-negative bacteria. The products responsible for O-antigen synthesis are encoded in the wbe region, which exhibits extensive genetic diversity. While heterogeneous O-antigens are observed within Vibrio species, characterization of these structures has been devoted almost exclusively to pathogens. Here, we investigate O-antigen diversity among coastal marine Vibrio splendidus-like isolates. The wbe region was first identified and characterized using the sequenced genomes of strains LGP32, 12B01 and Med222. These regions were genetically diverse, reflective of their expressed O-antigen. Additional isolates from physically distinct habitats in Plum Island Estuary (MA, USA), including within animal hosts and on suspended particles, were further characterized based on multilocus sequence analysis (MLSA) and O-antigen profiles. Results showed serotype diversity within an ecological setting. Among 48 isolates which were identical in three MLSA genes, 41 showed gpm genetic diversity, a gene closely linked to the wbe locus, and at least 12 expressed different O-antigen profiles further suggesting wbe genetic diversity. Our results demonstrate O-antigen hyper-variability among these environmental strains and suggest that frequent lateral gene transfer generates wbe extensive diversity among V. splendidus and its close relatives.

MeSH Terms
Antigenic Variation Base Sequence Gene Transfer, Horizontal Genetic Loci Genetic Variation Genome, Bacterial Massachusetts Molecular Sequence Data Multilocus Sequence Typing O Antigens/analysis,classification,genetics Open Reading Frames Phylogeny Sequence Analysis, DNA Serotyping Vibrio/genetics,immunology Water Microbiology
Chemicals
O Antigens
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Wildschutte Hans
Civil and Environmental Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA. [email protected]
Preheim Sarah P
Hernandez Yasiel
Polz Martin F
Article Info
Journal
Environmental microbiology
Abbr.
Environ Microbiol
ISSN
1462-2920
DOI
10.1111/j.1462-2920.2010.02274.x
Email
[email protected]
Published
2010-11-00
Pages
2977-87
Language
English
Region
England
NLM ID
100883692
Subset
IM
Grants
NIGMS NIH HHS · F32GM084640 · United States
Databases
GENBANK
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External Links
PubMed source DOI: 10.1111/j.1462-2920.2010.02274.x View journal details
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