Regulation of acetylcholine release from neuroblastoma x glioma hybrid cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 75 ·No. 3 ·1978-03-00 ·Pages 1314-8

McGee R, Simpson P, Christian C, Mata M, Nelson P, Nirenberg M

Abstract

Neuroblastoma x glioma NG108-15 hybrid cells exposed to N6, O2'-dibutyryladenosine 3':5'-cyclic monophosphate for several days release [3H]acetylcholine in response to serotonin, prostaglandin F2alpha, KCl, or veratridine. NG108-15 cells grown in the absence of dibutyrul cyclic AMP do not respond to an excitatory stimulus by releasing [3H]acetylcholine but can be shifted to a responsive state by treatment with dibutyryl cyclic AMP. Thus, the reactions that are required for acetylcholine release can be regulated in NG108-15 cells, thereby regulating the ability of cells to form synapses and the efficiency of synaptic communication.

MeSH Terms

Acetylcholine/metabolism Biological Transport/drug effects Bucladesine/pharmacology Calcium/pharmacology Cell Line Choline/metabolism Hybrid Cells Neurons/metabolism Potassium/pharmacology Prostaglandins/pharmacology Serotonin/pharmacology Synaptic Membranes/metabolism Tetrodotoxin/pharmacology Veratridine/pharmacology

Chemicals

Prostaglandins Serotonin Tetrodotoxin Bucladesine Veratridine Choline Acetylcholine Potassium Calcium

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

McGee R

Simpson P

Christian C

Mata M

Nelson P

Nirenberg M

References (16)

16 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.75.3.1314

Published

1978-03-00

Pages

1314-8

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC411461

Subset

External Links

PubMed source DOI: 10.1073/pnas.75.3.1314 PMC Full Text View journal details

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Regulation of acetylcholine release from neuroblastoma x glioma hybrid cells.

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