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PMID: 20725087 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Krüppel-like factor KLF9 regulates PPARγ transactivation at the middle stage of adipogenesis.

Cell death and differentiation ·Vol. 18 ·No. 2 ·2011-02-00 ·Pages 315-27

Pei H, Yao Y, Yang Y, Liao K, Wu JR

Abstract

Krüppel-like factors (KLFs) as a family of zinc-finger transcription factors involve in the regulation of many physiological processes. In these studies, KLF9 was characterized for its role in adipogenesis. The expression of KLF9 was markedly upregulated during the middle stage of 3T3-L1 adipocyte differentiation, and inhibition of KLF9 by RNAi impaired adipogenesis. Using promoter deletion and mutation analysis, we identified two KLF9-binding sites within the 0.6-kb region of the PPARγ2 proximal promoter, indicating that KLF9 interacts with the PPARγ2 promoter. Furthermore, we found that KLF9 could synergistically activate PPARγ2 promoter by directly interacting with C/EBPα. In addition, overexpression of PPARγ2 rescued the impairment of adipocyte differentiation induced by KLF9 knockdown, which supports that PPARγ2 is a downstream target of KLF9. Collectively, our results indicate KLF9 as a key pro-adipogenic transcription factor through regulation of PPARγ2 expression with C/EBPα at the middle stage of adipogenesis.

MeSH Terms
3T3-L1 Cells Adipocytes/metabolism Adipogenesis/genetics Animals CCAAT-Enhancer-Binding Protein-alpha/metabolism Cell Differentiation DNA Mutational Analysis Kruppel-Like Transcription Factors/genetics,metabolism,physiology Mice PPAR gamma/genetics,metabolism Promoter Regions, Genetic Protein Binding RNA Interference RNA, Small Interfering Rats Transcriptional Activation
Chemicals
CCAAT-Enhancer-Binding Protein-alpha Klf9 protein, mouse Kruppel-Like Transcription Factors PPAR gamma RNA, Small Interfering
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Pei H
Key Laboratory of Systems Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yue-Yang Road, Shanghai, China.
Yao Y
Yang Y
Liao K
Wu J-R
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Article Info
Journal
Cell death and differentiation
Abbr.
Cell Death Differ
ISSN
1476-5403
DOI
10.1038/cdd.2010.100
Published
2011-02-00
Epub
2010-00-20
Pages
315-27
Language
English
Region
England
NLM ID
9437445
PMCID
PMC3131894
Subset
IM
External Links
PubMed source DOI: 10.1038/cdd.2010.100 PMC Full Text View journal details
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