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PMID: 20798348 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, U.S. Gov't, Non-P.H.S.

Resolving postglacial phylogeography using high-throughput sequencing.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 107 ·No. 37 ·2010-09-14 ·Pages 16196-200

Emerson KJ, Merz CR, Catchen JM, Hohenlohe PA, Cresko WA, Bradshaw WE, Holzapfel CM

Abstract

The distinction between model and nonmodel organisms is becoming increasingly blurred. High-throughput, second-generation sequencing approaches are being applied to organisms based on their interesting ecological, physiological, developmental, or evolutionary properties and not on the depth of genetic information available for them. Here, we illustrate this point using a low-cost, efficient technique to determine the fine-scale phylogenetic relationships among recently diverged populations in a species. This application of restriction site-associated DNA tags (RAD tags) reveals previously unresolved genetic structure and direction of evolution in the pitcher plant mosquito, Wyeomyia smithii, from a southern Appalachian Mountain refugium following recession of the Laurentide Ice Sheet at 22,000-19,000 B.P. The RAD tag method can be used to identify detailed patterns of phylogeography in any organism regardless of existing genomic data, and, more broadly, to identify incipient speciation and genome-wide variation in natural populations in general.

MeSH Terms
Animals Appalachian Region Base Sequence Culicidae/chemistry,genetics Genetics, Population Mid-Atlantic Region Molecular Sequence Data New England Phylogeny Sequence Alignment Sequence Analysis, DNA/methods
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Emerson Kevin J
Center for Ecology and Evolutionary Biology, University of Oregon, Eugene, OR 97403-5289, USA.
Merz Clayton R
Catchen Julian M
Hohenlohe Paul A
Cresko William A
Bradshaw William E
Holzapfel Christina M
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
1091-6490
DOI
10.1073/pnas.1006538107
Published
2010-09-14
Epub
2010-00-26
Pages
16196-200
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC2941283
Subset
IM
Grants
NCRR NIH HHS · R01 RR020833 · United States
NIGMS NIH HHS · R24 GM079486 · United States
NIGMS NIH HHS · 1R24GM079486-01A1 · United States
NCRR NIH HHS · 3R01RR020833-04S1 · United States
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GENBANK
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External Links
PubMed source DOI: 10.1073/pnas.1006538107 PMC Full Text View journal details
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