Abstract
Pancreatic thread protein (PTP) forms double helical threads in the neutral pH range after purification, undergoing freely reversible, pH-dependent globule-fibril transformation. The purified bovine PTP consists on SDS gels of two carbohydrate-free polypeptide chains (Gross et al., 1985). Plasma desorption mass spectrometry and amino acid sequence analysis now confirm that bovine PTP contains two disulfide-bonded polypeptides, an A chain of 101 amino acid residues with a molecular weight of 11,073 and a B chain of 35 residues with a molecular weight of 3970. The intact protein exhibits a molecular weight of 15,036, agreeing greater than 99.9% with the molecular weight calculated from the sequence. The B chain sequence was determined by gas-phase Edman degradation of the intact polypeptide. The A chain sequence was determined from overlapping peptides generated by cleavage at lysyl, tryptophanyl, and aspartyl-prolyl residues. Based upon the bovine PTP cDNA structure, the two chains of the protein result from cleavage of a single polypeptide with removal of a dipeptide between the NH2-terminal A chain and COOH-terminal B chain. Comparison of bovine PTP with other proteins reveals significant structural relatedness with the single-chain homologues from human and rat pancreas and with the motif associated with Ca2(+)-dependent carbohydrate recognition domains. The physiological role of PTP has not yet been resolved. The protein is present in very high concentration in pancreatic secretion and it has been detected in brain lesions in Alzheimer's disease and Down syndrome and in regenerating rat pancreatic islets. The present results provide a firm protein base for ongoing molecular, physical-chemical, and structure-function studies of this unusual protein.
MeSH Terms
Amino Acid Sequence
Amino Acids/analysis
Animals
Calcium-Binding Proteins/chemistry
Cattle
Lithostathine
Mass Spectrometry
Molecular Sequence Data
Molecular Weight
Nerve Tissue Proteins
Peptide Fragments/isolation & purification
Protein Conformation
Sequence Homology, Nucleic Acid
Software
Chemicals
Amino Acids
Calcium-Binding Proteins
Lithostathine
Nerve Tissue Proteins
Peptide Fragments
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Cai L
W. Alton Jones Cell Science Center, Inc., Lake Placid, New York 12946.
Harris W R
Marshak D R
Gross J
Crabb J W
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