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PMID: 20859152 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Overexpression of karyopherin-2 in epithelial ovarian cancer and correlation with poor prognosis.

Obstetrics and gynecology ·Vol. 116 ·No. 4 ·2010-10-00 ·Pages 884-891

Zheng M, Tang L, Huang L, Ding H, Liao WT, Zeng MS, Wang HY

Abstract

To evaluate karyopherin 2 (KPNA2) as a biomarker for epithelial ovarian cancer. A candidate oncogene, KPNA2, was identified in gene microarray assays of epithelial ovarian cancer tissues compared with normal human ovarian surface epithelial tissues. Differences in expression were further validated by real-time polymerase chain reaction and Western blotting. KPNA2 expression patterns in epithelial ovarian cancer tissues were determined using immunohistochemistry and were compared with specific clinicopathologic features of the patient specimens analyzed. Factors associated with patient survival were also statistically analyzed. KPNA2 was found to be upregulated approximately eightfold in epithelial ovarian cancer tissues compared with human ovarian surface epithelial tissues, and overexpression was detected at the level of both transcription and translation. Immunohistochemical assays detected positive KPNA2 expression (++ or +++) in 50 of 102 (49.0%) epithelial ovarian cancer specimens, whereas negative KPNA2 expression (- or +) was observed in all of the human ovarian surface epithelial tissues analyzed. KPNA2 overexpression was also found to be significantly associated with specific histologic type, an advanced stage, a high histologic grade, and tumor recurrence (P<.05). The 5-year overall survival rate for KPNA2-negative compared with KPNA2-positive patients was 73.1% and 60.5%, respectively (P<.05). KPNA2 may play an important role in the development, differentiation, and carcinogenesis of epithelial ovarian cancer and therefore could be an indicator of poor prognosis for patients with epithelial ovarian cancer. II.

MeSH Terms
Adult Aged Biomarkers, Tumor/metabolism Blotting, Western Female Gene Expression Regulation, Neoplastic/physiology Humans Immunohistochemistry Kaplan-Meier Estimate Middle Aged Oligonucleotide Array Sequence Analysis Ovarian Neoplasms/metabolism,mortality,pathology Prognosis Reverse Transcriptase Polymerase Chain Reaction Young Adult alpha Karyopherins/metabolism
Chemicals
Biomarkers, Tumor KPNA2 protein, human alpha Karyopherins
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Zheng Min
From the State Key Laboratory of Oncology in Southern China and the Department of Gynecology, Cancer Center, Sun Yat-sen University, Guangzhou, Guangdong, China.
Tang Li
Huang Long
Ding Hui
Liao Wen-Ting
Zeng Mu-Sheng
Wang Hui-Yun
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Article Info
Journal
Obstetrics and gynecology
Abbr.
Obstet Gynecol
ISSN
1873-233X
DOI
10.1097/AOG.0b013e3181f104ce
Published
2010-10-00
Pages
884-891
Language
English
Region
United States
NLM ID
0401101
Subset
IM
External Links
PubMed source DOI: 10.1097/AOG.0b013e3181f104ce
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