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PMID: 20889529 Published · ppublish English Journal Article Research Support, N.I.H., Extramural

Effect of ICSI on gene expression and development of mouse preimplantation embryos.

Human reproduction (Oxford, England) ·Vol. 25 ·No. 12 ·2010-12-00 ·Pages 3012-24

Giritharan G, Li MW, Di Sebastiano F, De Sebastiano F, Esteban FJ, Horcajadas JA, Lloyd KC, Donjacour A, Maltepe E, Rinaudo PF

Abstract

In vitro culture (IVC) and IVF of preimplantation mouse embryos are associated with changes in gene expression. It is however not known whether ICSI has additional effects on the transcriptome of mouse blastocysts. We compared gene expression and development of mouse blastocysts produced by ICSI and cultured in Whitten's medium (ICSI(WM)) or KSOM medium with amino acids (ICSI(KSOMaa)) with control blastocysts flushed out of the uterus on post coital Day 3.5 (in vivo). In addition, we compared gene expression in embryos generated by IVF or ICSI using WM. Global pattern of gene expression was assessed using the Affymetrix 430 2.0 chip. Blastocysts from ICSI fertilization have a reduction in the number of trophoblastic and inner cell mass cells compared with embryos generated in vivo. Approximately 1000 genes are differentially expressed between ICSI blastocyst and in vivo blastocysts; proliferation, apoptosis and morphogenetic pathways are the most common pathways altered after IVC. Unexpectedly, expression of only 41 genes was significantly different between embryo cultured in suboptimal conditions (WM) or optimal conditions (KSOM(aa)). Our results suggest that fertilization by ICSI may play a more important role in shaping the transcriptome of the developing mouse embryo than the culture media used.

MeSH Terms
Animals Blastocyst/metabolism DNA-Binding Proteins/biosynthesis Embryo Culture Techniques Embryonic Development Female Gene Expression Profiling Gene Expression Regulation, Developmental Histone Deacetylase 6 Histone Deacetylases/biosynthesis Histone-Lysine N-Methyltransferase Mice Myeloid-Lymphoid Leukemia Protein/biosynthesis Protein Array Analysis Sperm Injections, Intracytoplasmic Transcription Factors/biosynthesis
Chemicals
DNA-Binding Proteins Smarca1 protein, mouse Transcription Factors Myeloid-Lymphoid Leukemia Protein Histone-Lysine N-Methyltransferase Kmt2b protein, mouse Hdac6 protein, mouse Histone Deacetylase 6 Histone Deacetylases
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Giritharan G
Department of Obstetric, Gynecology and Reproductive Sciences, University of California, San Francisco, San Francisco, CA 94143, USA.
Li M W
Di Sebastiano F
De Sebastiano F
Esteban F J
Horcajadas J A
Lloyd K C K
Donjacour A
Maltepe E
Rinaudo P F
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Article Info
Journal
Human reproduction (Oxford, England)
Abbr.
Hum Reprod
ISSN
1460-2350
DOI
10.1093/humrep/deq266
Published
2010-12-00
Epub
2010-00-01
Pages
3012-24
Language
English
Region
England
NLM ID
8701199
PMCID
PMC2989873
Subset
IM
Grants
NCI NIH HHS · P30 CA093373 · United States
NICHD NIH HHS · R01 HD062803 · United States
NIH HHS · U42 OD012210 · United States
NICHD NIH HHS · 1U54HD055764 · United States
Corrections
ErratumIn
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External Links
PubMed source DOI: 10.1093/humrep/deq266 PMC Full Text View journal details
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