Competitive antagonism by phentolamine of responses to biogenic amines and the transmitter at a neuroglandular junction.

The Journal of physiology ·Vol. 279 ·1978-06-00 ·Pages 473-89

Bowser-Riley F, House CR, Smith RK

Abstract

1. A quantitative study has been made of phentolamine's inhibition of the electrical and secretory responses of the isolated salivary glands of Nauphoeta cinerea Olivier to nerve stimulation and bath applications of agonists. 2. The results suggested that phentolamine is a competitive antagonist having an affinity constant of about 1 micrometer-1 for the receptors for dopamine, noradrenaline, adrenaline and the neurotransmitter. 3. Phentolamine also inhibited responses to 5-hydroxytryptamine in a manner seemingly more complex than competitive antagonism. Attempts to estimate the affinity constant gave values of about 0.08 and 0.015 microgram-1 for inhibition of the secretory and electrical responses respectively. 4. This investigation showed that phentolamine discriminates between two kinds of receptor in this gland, one binding 5-hydroxytryptamine and the other combining with catecholamines and the neurotransmitter.

MeSH Terms

Animals Biogenic Amines/antagonists & inhibitors Cockroaches/physiology Dopamine Antagonists Dose-Response Relationship, Drug Female In Vitro Techniques Male Membrane Potentials/drug effects Phentolamine/pharmacology Salivary Glands/innervation Salivation/drug effects Secretory Rate/drug effects Serotonin Antagonists Synaptic Transmission/drug effects

Chemicals

Biogenic Amines Dopamine Antagonists Serotonin Antagonists Phentolamine

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Bowser-Riley F

House C R

Smith R K

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25 references, click to expand

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Article Info

Journal

The Journal of physiology

Abbr.

J Physiol

ISSN

0022-3751

DOI

10.1113/jphysiol.1978.sp012357

Published

1978-06-00

Pages

473-89

Language

English

Region

England

NLM ID

0266262

PMCID

PMC1282628

Subset

External Links

PubMed source DOI: 10.1113/jphysiol.1978.sp012357 PMC Full Text View journal details

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Competitive antagonism by phentolamine of responses to biogenic amines and the transmitter at a neuroglandular junction.

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