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PMID: 20927106 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Jasmonate perception by inositol-phosphate-potentiated COI1-JAZ co-receptor.

Nature ·Vol. 468 ·No. 7322 ·2010-11-18 ·Pages 400-5

Sheard LB, Tan X, Mao H, Withers J, Ben-Nissan G, Hinds TR, Kobayashi Y, Hsu FF, Sharon M, Browse J, He SY, Rizo J, Howe GA, Zheng N

Abstract

Jasmonates are a family of plant hormones that regulate plant growth, development and responses to stress. The F-box protein CORONATINE INSENSITIVE 1 (COI1) mediates jasmonate signalling by promoting hormone-dependent ubiquitylation and degradation of transcriptional repressor JAZ proteins. Despite its importance, the mechanism of jasmonate perception remains unclear. Here we present structural and pharmacological data to show that the true Arabidopsis jasmonate receptor is a complex of both COI1 and JAZ. COI1 contains an open pocket that recognizes the bioactive hormone (3R,7S)-jasmonoyl-l-isoleucine (JA-Ile) with high specificity. High-affinity hormone binding requires a bipartite JAZ degron sequence consisting of a conserved α-helix for COI1 docking and a loop region to trap the hormone in its binding pocket. In addition, we identify a third critical component of the jasmonate co-receptor complex, inositol pentakisphosphate, which interacts with both COI1 and JAZ adjacent to the ligand. Our results unravel the mechanism of jasmonate perception and highlight the ability of F-box proteins to evolve as multi-component signalling hubs.

MeSH Terms
Amino Acid Sequence Amino Acids/chemistry,metabolism Arabidopsis/chemistry,metabolism Arabidopsis Proteins/chemistry,metabolism Binding Sites Crystallography, X-Ray Cyclopentanes/chemistry,metabolism F-Box Proteins/chemistry,metabolism Indenes/chemistry,metabolism Inositol Phosphates/metabolism Isoleucine/analogs & derivatives,chemistry,metabolism Models, Molecular Molecular Sequence Data Oxylipins/chemistry,metabolism Peptide Fragments/chemistry,metabolism Plant Growth Regulators/chemistry,metabolism Protein Binding Protein Structure, Tertiary Repressor Proteins/chemistry,metabolism Signal Transduction
Chemicals
ASK1 protein, Arabidopsis Amino Acids Arabidopsis Proteins COI1 protein, Arabidopsis Cyclopentanes F-Box Proteins Indenes Inositol Phosphates JAZ1 protein, Arabidopsis JAZ6 protein, Arabidopsis Oxylipins Peptide Fragments Plant Growth Regulators Repressor Proteins jasmonoyl-isoleucine Isoleucine inositol pentaphosphate coronatine jasmonic acid
Authors & Affiliations
14 authors, click to expand affiliations / ORCID
Sheard Laura B
Department of Pharmacology, Box 357280, University of Washington, Seattle, Washington 98195, USA.
Tan Xu
Mao Haibin
Withers John
Ben-Nissan Gili
Hinds Thomas R
Kobayashi Yuichi
Hsu Fong-Fu
Sharon Michal
Browse John
He Sheng Yang
Rizo Josep
Howe Gregg A
Zheng Ning
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Article Info
Journal
Nature
Abbr.
Nature
ISSN
1476-4687
DOI
10.1038/nature09430
Published
2010-11-18
Epub
2010-00-06
Pages
400-5
Language
English
Region
England
NLM ID
0410462
PMCID
PMC2988090
Subset
IM
Grants
NCI NIH HHS · R01 CA107134 · United States
NIGMS NIH HHS · R01 GM057795-12 · United States
NIGMS NIH HHS · T32 GM07270 · United States
NIAID NIH HHS · R01 AI068718-04 · United States
NIGMS NIH HHS · T32 GM007270 · United States
NIDDK NIH HHS · P30 DK056341-10 · United States
Howard Hughes Medical Institute · United States
NCI NIH HHS · R01 CA107134-07 · United States
NIAID NIH HHS · R01 AI068718 · United States
NIAID NIH HHS · R01AI068718 · United States
NIGMS NIH HHS · R01GM57795 · United States
NIDDK NIH HHS · P30 DK056341 · United States
NIGMS NIH HHS · R01 GM057795 · United States
Databases
PDB
3OGK, 3OGL, 3OGM
External Links
PubMed source DOI: 10.1038/nature09430 PMC Full Text View journal details
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