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PMID: 20938544 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, U.S. Gov't, P.H.S.

A new chemotaxis device for cell migration studies.

Integrative biology : quantitative biosciences from nano to macro ·Vol. 2 ·No. 11-12 ·2010-11-00 ·Pages 696-706

Raja WK, Gligorijevic B, Wyckoff J, Condeelis JS, Castracane J

Abstract

This study presents the design and optimization for in vitro use of a new versatile chemotaxis device called the NANIVID (NANo IntraVital Imaging Device), developed using advanced nano/micro fabrication techniques. The device is fabricated using microphotolithographic techniques and two substrates are bonded together using a thin polymer layer creating a sealed device with one outlet. The main structure of the device consists of two Pyrex substrates: an etched chemoattractant reservoir and a top cover, with a final size of 0.2 × 2 × 3 mm. This reservoir contains a hydrogel blend with EGF which diffuses out through a small (∼9.10(3)μm(2)) outlet. This reservoir sustains a steady release of growth factor into the surrounding environment for several hours establishing a consistent concentration gradient from the device. The focus of this study was to design and optimize the new device for cell chemotaxis studies in breast cancer cells in cell culture. Our results show that we have created a flexible, cheap, miniature and autonomous chemotaxis device and demonstrate its usefulness in 2D and 3D cell culture. We also provide preliminary data for use of the device in vivo.

MeSH Terms
Animals Biomedical Engineering Breast Neoplasms/physiopathology Cell Line, Tumor Cell Movement/drug effects,physiology Chemotaxis/drug effects,physiology Epidermal Growth Factor/pharmacology Equipment Design Female Humans Hydrogels Mammary Neoplasms, Experimental/physiopathology Microfluidic Analytical Techniques/instrumentation Nanotechnology Rats
Chemicals
Hydrogels Epidermal Growth Factor
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Raja Waseem Khan
College of Nanoscale Science and Engineering, University at Albany, SUNY, Albany, NY, USA.
Gligorijevic Bojana
Wyckoff Jeff
Condeelis John S
Castracane James
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Article Info
Journal
Integrative biology : quantitative biosciences from nano to macro
Abbr.
Integr Biol (Camb)
ISSN
1757-9708
DOI
10.1039/c0ib00044b
Published
2010-11-00
Epub
2010-00-12
Pages
696-706
Language
English
Region
England
NLM ID
101478378
PMCID
PMC4026270
Subset
IM
Grants
NCI NIH HHS · U54 CA126511 · United States
PHS HHS · UO1-1105490 · United States
NCI NIH HHS · U54-CA126511 · United States
NCI NIH HHS · BC075554 · United States
External Links
PubMed source DOI: 10.1039/c0ib00044b PMC Full Text View journal details
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