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PMID: 209408 Published · ppublish English Journal Article

Localization of the 5' terminus of late SV40 mRNA.

Nucleic acids research ·Vol. 5 ·No. 7 ·1978-07-00 ·Pages 2359-71

Haegeman G, Fiers W

Abstract

A cap-containing oligonucleotide has been isolated from a T1 ribonuclease hydrolysate of total Simian Virus 40-specific late RNA and its structure has been determined as 7mG(5')ppp(5') mAmpU(m)pUp(Up,Cp)ApGp. This oligonucleotide constitutes the major 5' terminus of both late mRNA species, 16S and 19S. Assuming that viral mRNA "caps" are derived from a 5'-terminal triphosphate or diphosphate, these results mean that late transcription is (mainly) initiated at nucleotide L 308 (numbering system of Simian Virus 40 DNA, cf. Fiers et al. (1978) Nature, 273, 113-120). The cap is followed by a contiguous leader sequence which is at least 194 nucleotides long.

MeSH Terms
Base Sequence Guanosine Triphosphate/analogs & derivatives,isolation & purification Oligonucleotides/isolation & purification Oligoribonucleotides/isolation & purification RNA, Messenger/analysis,genetics Simian virus 40/genetics Transcription, Genetic
Chemicals
Oligonucleotides Oligoribonucleotides RNA, Messenger Guanosine Triphosphate
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Haegeman G
Fiers W
References (30)
30 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/5.7.2359
Published
1978-07-00
Pages
2359-71
Language
English
Region
England
NLM ID
0411011
PMCID
PMC342169
Subset
IM
Databases
GENBANK
J02400, J02402, J02403, J02406, J02407, J02408, J02409, J02410, V01380
External Links
PubMed source DOI: 10.1093/nar/5.7.2359 PMC Full Text View journal details
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