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PMID: 20947016 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Crystal structure of group II chaperonin in the open state.

Structure (London, England : 1993) ·Vol. 18 ·No. 10 ·2010-10-13 ·Pages 1270-9

Huo Y, Hu Z, Zhang K, Wang L, Zhai Y, Zhou Q, Lander G, Zhu J, He Y, Pang X, Xu W, Bartlam M, Dong Z, Sun F

Abstract

Thermosomes are group II chaperonins responsible for protein refolding in an ATP-dependent manner. Little is known regarding the conformational changes of thermosomes during their functional cycle due to a lack of high-resolution structure in the open state. Here, we report the first complete crystal structure of thermosome (rATcpnβ) in the open state from Acidianus tengchongensis. There is a ∼30° rotation of the apical and lid domains compared with the previous closed structure. Besides, the structure reveals a conspicuous hydrophobic patch in the lid domain, and residues locating in this patch are conserved across species. Both the closed and open forms of rATcpnβ were also reconstructed by electron microscopy (EM). Structural fitting revealed the detailed conformational change from the open to the closed state. Structural comparison as well as protease K digestion indicated only ATP binding without hydrolysis does not induce chamber closure of thermosome.

MeSH Terms
Acidianus/metabolism Adenosine Diphosphate/chemistry,metabolism Adenosine Triphosphate/chemistry,metabolism Archaeal Proteins/chemistry,genetics,metabolism Cloning, Molecular Cryoelectron Microscopy Crystallization Electrophoresis, Polyacrylamide Gel Models, Molecular Protein Binding Protein Conformation Protein Folding Protein Structure, Tertiary Thermosomes/chemistry,genetics,metabolism X-Ray Diffraction
Chemicals
Archaeal Proteins Adenosine Diphosphate Adenosine Triphosphate Thermosomes
Authors & Affiliations
14 authors, click to expand affiliations / ORCID
Huo Yanwu
National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China.
Hu Zhongjun
Zhang Kai
Wang Li
Zhai Yujia
Zhou Qiangjun
Lander Gabe
Zhu Jiang
He Yongzhi
Pang Xiaoyun
Xu Wei
Bartlam Mark
Dong Zhiyang
Sun Fei
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Article Info
Journal
Structure (London, England : 1993)
Abbr.
Structure
ISSN
1878-4186
DOI
10.1016/j.str.2010.07.009
Published
2010-10-13
Pages
1270-9
Language
English
Region
United States
NLM ID
101087697
PMCID
PMC3048791
Subset
IM
Grants
NCRR NIH HHS · P41 RR017573 · United States
NCRR NIH HHS · P41 RR17573 · United States
Databases
PDB
3KO1
Corrections
CommentIn
PMID: 20947006
External Links
PubMed source DOI: 10.1016/j.str.2010.07.009 PMC Full Text View journal details
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