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PMID: 2102855 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Alcohol dehydrogenase gene expression in potato following elicitor and stress treatment.

Plant molecular biology ·Vol. 14 ·No. 5 ·1990-05-00 ·Pages 775-83

Matton DP, Constabel P, Brisson N

Abstract

A cDNA clone corresponding to a mRNA that rapidly accumulates during the hypersensitive-like response induced by elicitor treatment of potato (Solanum tuberosum L.) tuber was characterized. The clone encodes a polypeptide (Mr = 41,097) having 83%-85% amino acid identity with known plant alcohol dehydrogenase sequences (ADH; EC 1.1.1.1). The identity of the clone was confirmed by measuring the ADH enzyme activity in extracts of Escherichia coli transformed with the cDNA clone. In potato tuber disks, a wide range of stresses, including treatment with fatty acid elicitors, salicylic acid, UV light and anaerobiosis, was shown to induce accumulation of Adh transcripts. In stems, a high constitutive level of Adh transcripts could be detected in 4-week old plants, but not in 8-week old plants. However, the mRNA could be induced to accumulate in stems of 8-week old plants by treatment with arachidonic acid elicitor or by anaerobiosis. Induction in leaves was also obtained during anaerobiosis and after treatment with a Phytophthora infestans mycelial homogenate.

MeSH Terms
Alcohol Dehydrogenase/genetics Amino Acid Sequence Base Sequence DNA/genetics Fatty Acids/pharmacology Gene Expression/drug effects Genes, Plant Molecular Sequence Data Plants/enzymology,genetics RNA, Messenger/genetics Solanum tuberosum/enzymology,genetics Transcription, Genetic
Chemicals
Fatty Acids RNA, Messenger DNA Alcohol Dehydrogenase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Matton D P
Department of Biochemistry, Université de Montréal, Canada.
Constabel P
Brisson N
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Article Info
Journal
Plant molecular biology
Abbr.
Plant Mol Biol
ISSN
0167-4412
DOI
10.1007/BF00016510
Published
1990-05-00
Pages
775-83
Language
English
Region
Netherlands
NLM ID
9106343
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00016510 View journal details
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