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PMID: 2105505 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Detection of antibody against antigen expressed by molecularly cloned hepatitis C virus cDNA: application to diagnosis and blood screening for posttransfusion hepatitis.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 87 ·No. 3 ·1990-02-00 ·Pages 983-7

Miyamura T, Saito I, Katayama T, Kikuchi S, Tateda A, Houghton M, Choo QL, Kuo G

Abstract

A cDNA clone has been derived from the plasma of a chimpanzee with chronic non-A, non-B viral hepatitis (NANBH). We have assayed for antibodies reacting with the encoded antigen in sera from posttransfusion hepatitis patients (643 samples from 23 patients) and their corresponding donors collected during the past 10 years in Japan. The antibody was detected in 15 out of 17 (88.2%) posttransfusion NANBH (PT-NANBH) patients whose sera over time displayed multiple alanine aminotransferase (ALT) peaks. In general, the antibody was detected after several peaks of serum ALT elevations and, once detected, it persisted for years. In contrast to the patients of chronic hepatitis, the antibody was barely detected in patients with a single episode of ALT elevation (1 out of 6). Of the 15 well-defined cases of PT-NANBH that showed multiple ALT peaks and hepatitis C virus seroconversions, 11 (73.3%) were shown to be transfused with at least one unit of blood positive for the antibody. The retrospective analysis showed that all tested donor blood found to be positive for the antibody had been transfused to recipients who afterwards developed NANBH. These data strongly suggest that the cloned cDNA originated from an etiological agent of NANBH termed the hepatitis C virus. Furthermore, the present study demonstrates that had the screening been done with the anti-hepatitis C virus assay, 11 out of 17 (64.7%) cases of chronic PT-NANBH and 1 out of 6 (16.6%) acute PT-NANBH would have been prevented. The antibody assay thus can be used for diagnosis and blood screening for PT-NANBH.

MeSH Terms

Adult Aged Antigens, Viral/genetics,immunology DNA, Viral/genetics Female Hepatitis Antibodies/analysis Hepatitis C/diagnosis,etiology,immunology Hepatitis Viruses/genetics,immunology Hepatitis, Viral, Human/diagnosis Humans Male Middle Aged Transfusion Reaction

Chemicals

Antigens, Viral DNA, Viral Hepatitis Antibodies

Authors & Affiliations

8 authors, click to expand affiliations / ORCID

Miyamura T

Department of Enteroviruses, National Institute of Health, Tokyo, Japan.

Saito I

Katayama T

Kikuchi S

Tateda A

Houghton M

Choo Q L

Kuo G

References (14)

14 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.87.3.983

Published

1990-02-00

Pages

983-7

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC53394

Subset

External Links

PubMed source DOI: 10.1073/pnas.87.3.983 PMC Full Text View journal details

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Detection of antibody against antigen expressed by molecularly cloned hepatitis C virus cDNA: application to diagnosis and blood screening for posttransfusion hepatitis.

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