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PMID: 2106486 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Production of tumour necrosis factor by mastocytoma P815 cells.

Immunology ·Vol. 69 ·No. 2 ·1990-02-00 ·Pages 312-5

Ohno I, Tanno Y, Yamauchi K, Takishima T

Abstract

P815, a transformed mouse mastocytoma cell line, produced and released cytotoxic factors after stimulation with phorbol 12-myristate 13-acetate (PMA), but not with lipopolysaccharide (LPS), calcium ionophore A23187 and IgE receptor triggering. The cytotoxic activity was reduced 60% by antibodies to mouse tumour necrosis factor (TNF). In addition, we demonstrated that TNF mRNA was already expressed under normal culture conditions and that it increased after stimulation with PMA. Although it was unknown whether factors other than TNF were lymphotoxin or some other unknown factors, it has been suggested that mast cells have cytotoxic activity, and that they contribute to inflammatory response through the release of TNF, which has a wide range of biological activity.

MeSH Terms
Animals Cell Line, Transformed Cytotoxicity, Immunologic Gene Expression Mast-Cell Sarcoma/immunology,metabolism Mice Tetradecanoylphorbol Acetate Tumor Cells, Cultured/drug effects,metabolism Tumor Necrosis Factor-alpha/biosynthesis,genetics
Chemicals
Tumor Necrosis Factor-alpha Tetradecanoylphorbol Acetate
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Ohno I
First Department of Internal Medicine, Tohoku University School of Medicine, Sendai, Japan.
Tanno Y
Yamauchi K
Takishima T
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Article Info
Journal
Immunology
Abbr.
Immunology
ISSN
0019-2805
Published
1990-02-00
Pages
312-5
Language
English
Region
England
NLM ID
0374672
PMCID
PMC1385607
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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