Home LiteratureArticle Details
PMID: 2106512 Published · ppublish English Comparative Study Journal Article

Bacillopeptidase F of Bacillus subtilis: purification of the protein and cloning of the gene.

Journal of bacteriology ·Vol. 172 ·No. 3 ·1990-03-00 ·Pages 1470-7

Sloma A, Rufo GA, Rudolph CF, Sullivan BJ, Theriault KA, Pero J

Abstract

We have purified a minor extracellular serine protease from Bacillus subtilis. Characterization of this enzyme indicated that it was most likely the previously reported enzyme bacillopeptidase F. The amino-terminal sequence of the purified protein was determined, and a "guess-mer" oligonucleotide hybridization probe was constructed on the basis of that sequence. This probe was used to identify and clone the structural gene (bpr) for bacillopeptidase F. The deduced amino acid sequence for the mature protein (496 amino acids) was preceded by a putative signal sequence of 30 residues and a putative propeptide region of 164 amino acids. The bpr gene mapped near pyrD on the chromosome and was not required for growth or sporulation.

MeSH Terms
Amino Acid Sequence Bacillus subtilis/enzymology,genetics Base Sequence Blotting, Southern Chromatography, Affinity Chromatography, High Pressure Liquid Chromosome Mapping Chromosomes, Bacterial Cloning, Molecular Genes, Bacterial Genotype Kinetics Molecular Sequence Data Nucleic Acid Hybridization Oligonucleotide Probes Plasmids Restriction Mapping Sequence Homology, Nucleic Acid Serine Endopeptidases/genetics,isolation & purification,metabolism
Chemicals
Oligonucleotide Probes Serine Endopeptidases bacillopeptidase F
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Sloma A
BioTechnica International, Inc., Cambridge, Massachusetts 02140.
Rufo G A
Rudolph C F
Sullivan B J
Theriault K A
Pero J
References (30)
30 references, click to expand
  1. REQUIREMENTS FOR TRANSFORMATION IN BACILLUS SUBTILIS.
    J Bacteriol. 1961 May;81(5):741-6 PMID: 16561900
  2. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  3. Structure and biosynthesis of prokaryotic glycoproteins.
    Biochimie. 1988 Nov;70(11):1493-504 PMID: 3149518
  4. Extracellular enzyme synthesis in the genus Bacillus.
    Bacteriol Rev. 1977 Sep;41(3):711-53 PMID: 334155
  5. Construction of a Bacillus subtilis double mutant deficient in extracellular alkaline and neutral proteases.
    J Bacteriol. 1984 Oct;160(1):442-4 PMID: 6434524
  6. Transformation and transduction in recombination-defective mutants of Bacillus subtilis.
    J Bacteriol. 1967 Jun;93(6):1925-37 PMID: 4960898
  7. Gene encoding a minor extracellular protease in Bacillus subtilis.
    J Bacteriol. 1988 Dec;170(12):5557-63 PMID: 3142851
  8. Construction of a cloning site near one end of Tn917 into which foreign DNA may be inserted without affecting transposition in Bacillus subtilis or expression of the transposon-borne erm gene.
    Plasmid. 1984 Jul;12(1):1-9 PMID: 6093169
  9. Gene encoding a novel extracellular metalloprotease in Bacillus subtilis.
    J Bacteriol. 1990 Feb;172(2):1024-9 PMID: 2105291
  10. Catabolic repression of bacterial sporulation.
    Proc Natl Acad Sci U S A. 1965 Sep;54(3):704-11 PMID: 4956288
  11. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  12. Improved estimation of secondary structure in ribonucleic acids.
    Nat New Biol. 1973 Nov 14;246(150):40-1 PMID: 4519026
  13. Characterization of Staphylococcus aureus plasmids introduced by transformation into Bacillus subtilis.
    J Bacteriol. 1978 Apr;134(1):318-29 PMID: 418061
  14. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  15. Protease from Staphyloccus aureus.
    Methods Enzymol. 1976;45:469-75 PMID: 1012010
  16. 'DNA Strider': a 'C' program for the fast analysis of DNA and protein sequences on the Apple Macintosh family of computers.
    Nucleic Acids Res. 1988 Mar 11;16(5):1829-36 PMID: 2832831
  17. Cloning of the neutral protease gene of Bacillus subtilis and the use of the cloned gene to create an in vitro-derived deletion mutation.
    J Bacteriol. 1984 Oct;160(1):15-21 PMID: 6090407
  18. Replacement of the Bacillus subtilis subtilisin structural gene with an In vitro-derived deletion mutation.
    J Bacteriol. 1984 May;158(2):411-8 PMID: 6427178
  19. A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
    Nucleic Acids Res. 1979 Nov 24;7(6):1513-23 PMID: 388356
  20. Bacillopeptidase F: two forms of a glycoprotein serine protease from Bacillus subtilis 168.
    J Bacteriol. 1983 Jul;155(1):145-52 PMID: 6408058
  21. Production of two proteolytic enzymes by a transformable strain of Bacillus subtilis.
    Arch Biochem Biophys. 1968 Nov;128(2):442-55 PMID: 4972346
  22. Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene.
    Proc Natl Acad Sci U S A. 1975 Oct;72(10):3961-5 PMID: 1105573
  23. Thermosensitive, extracellular neutral proteases in Bacillus subtilis: isolation, characterization, and genetics.
    J Bacteriol. 1979 Aug;139(2):583-90 PMID: 37241
  24. Cloning and sequencing of the major intracellular serine protease gene of Bacillus subtilis.
    J Bacteriol. 1986 Jul;167(1):110-6 PMID: 3087947
  25. Fate of transforming DNA following uptake by competent Bacillus subtilis. I. Formation and properties of the donor-recipient complex.
    J Mol Biol. 1971 Mar 14;56(2):209-21 PMID: 4994568
  26. High resolution two-dimensional electrophoresis of proteins.
    J Biol Chem. 1975 May 25;250(10):4007-21 PMID: 236308
  27. Construction of a kit of reference strains for rapid genetic mapping in Bacillus subtilis 168.
    Appl Environ Microbiol. 1977 Apr;33(4):989-93 PMID: 405929
  28. A putative signal peptidase recognition site and sequence in eukaryotic and prokaryotic signal peptides.
    J Mol Biol. 1983 Jun 25;167(2):391-409 PMID: 6345794
  29. [Purification and properties of an esterase excreted during sporulation of Bacillus subtilis].
    Biochimie. 1975;57(1):9-16 PMID: 238664
  30. Isolation and characterization of a novel extracellular metalloprotease from Bacillus subtilis.
    J Bacteriol. 1990 Feb;172(2):1019-23 PMID: 2105290
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.172.3.1470-1477.1990
Published
1990-03-00
Pages
1470-7
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC208622
Subset
IM
Databases
GENBANK
M29035
Corrections
ErratumIn
-
External Links
PubMed source DOI: 10.1128/jb.172.3.1470-1477.1990 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2