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PMID: 2106664 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Analysis of the rat JE gene promoter identifies an AP-1 binding site essential for basal expression but not for TPA induction.

Nucleic acids research ·Vol. 18 ·No. 1 ·1990-01-11 ·Pages 23-34

Timmers HT, Pronk GJ, Bos JL, van der Eb AJ

Abstract

We have cloned the immediate-early serum-reponsive JE gene from the rat in order to study the regulation of this gene. We show that sequences of the JE promoter region confer serum-inducibility to a reporter gene. Analysis of the promoter in transient assays reveals that: i) the -141/-88 region is required for the response to the phorbol ester TPA, ii) the -70/-38 region is essential for basal activity. This latter region harbors the sequence TGACTCC, which resembles the consensus site for AP-1 binding, TGACTCA. DNA-protein binding assays indicate that the JE AP-1 site and the consensus AP-1 site have an overlapping but not identical binding spectrum for AP-1 proteins. Our data suggest that the inability of some AP-1 sites to respond to TPA is caused by subtle differences in affinity for AP-1 proteins.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Chemokine CCL2 Cloning, Molecular DNA-Binding Proteins/physiology Gene Expression Regulation/drug effects Glycoproteins/genetics Molecular Sequence Data Promoter Regions, Genetic Proto-Oncogene Proteins c-jun RNA Splicing Rats Tetradecanoylphorbol Acetate/pharmacology Transcription Factors/physiology Transcription, Genetic
Chemicals
Chemokine CCL2 DNA-Binding Proteins Glycoproteins Proto-Oncogene Proteins c-jun Transcription Factors Tetradecanoylphorbol Acetate
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Timmers H T
Laboratory for Molecular Carcinogenesis, Sylvius Laboratories, University of Leiden, The Netherlands.
Pronk G J
Bos J L
van der Eb A J
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/18.1.23
Published
1990-01-11
Pages
23-34
Language
English
Region
England
NLM ID
0411011
PMCID
PMC330199
Subset
IM
Databases
GENBANK
X17053
External Links
PubMed source DOI: 10.1093/nar/18.1.23 PMC Full Text View journal details
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