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PMID: 2106683 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The SpoOA protein of Bacillus subtilis is a repressor of the abrB gene.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 87 ·No. 5 ·1990-03-00 ·Pages 1801-5

Strauch M, Webb V, Spiegelman G, Hoch JA

Abstract

The spoOA gene of Bacillus subtilis is critical for the initial stages in the developmental cycle leading to the formation of an endospore. We show that one function of the SpoOA protein is to negatively regulate another regulatory locus, abrB, which controls the expression of many genes associated with the onset of sporulation. Purified SpoOA protein binds to a specific region of the abrB promoter and functions as a repressor of transcription in an in vitro assay. The binding of the SpoOA protein is independent of the binding of the AbrB protein, which is known to autoregulate its expression. This independence mirrors the temporal sequence of events in abrB control.

MeSH Terms
Bacillus subtilis/genetics,metabolism Bacterial Proteins/genetics,metabolism Base Sequence DNA, Bacterial/genetics,metabolism DNA-Binding Proteins/genetics Genes, Bacterial Kinetics Molecular Sequence Data Promoter Regions, Genetic Repressor Proteins/metabolism Spores, Bacterial/metabolism Transcription Factors/genetics,metabolism Transcription, Genetic
Chemicals
AbrB protein, Bacillus subtilis Bacterial Proteins DNA, Bacterial DNA-Binding Proteins Repressor Proteins Spo0A protein, Bacillus subtilis Transcription Factors
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Strauch M
Department of Molecular and Experimental Medicine, Research Institute of Scripps Clinic, La Jolla, CA 92037.
Webb V
Spiegelman G
Hoch J A
References (28)
28 references, click to expand
  1. The transition state transcription regulator AbrB of Bacillus subtilis is autoregulated during vegetative growth.
    Mol Microbiol. 1989 Sep;3(9):1203-9 PMID: 2507867
  2. The transition state transcription regulator abrB of Bacillus subtilis is a DNA binding protein.
    EMBO J. 1989 May;8(5):1615-21 PMID: 2504584
  3. Phenotypic reversion in some early blocked sporulation mutants of Bacillus subtilis: isolation and phenotype identification of partial revertants.
    J Bacteriol. 1971 Oct;108(1):241-7 PMID: 4331293
  4. Phenotypes of pleiotropic-negative sporulation mutants of Bacillus subtilis.
    J Bacteriol. 1973 Sep;115(3):1063-70 PMID: 4199504
  5. Pleiotropic nature of bacteriophage tolerant mutants obtained in early-blocked asporogenous mutants of Bacillus subtilis 168.
    Mol Gen Genet. 1973 Aug 10;124(2):97-106 PMID: 4201042
  6. Genetics of bacterial sporulation.
    Adv Genet. 1976;18:69-98 PMID: 821319
  7. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  8. Nucleotide sequence of sporulation locus spoIIA in Bacillus subtilis.
    J Gen Microbiol. 1984 Aug;130(8):2147-53 PMID: 6088674
  9. Identification of the transcriptional suppressor sof-1 as an alteration in the spo0A protein.
    J Bacteriol. 1985 Feb;161(2):552-5 PMID: 2981817
  10. Early blocked asporogenous mutants of Bacillus subtilis 168. I. Isolation and characterization of mutants resistant to antibiotic(s) produced by sporulating Bacillus subtilis 168.
    Mol Gen Genet. 1971;112(2):104-9 PMID: 5000489
  11. Phenotypic reversion in some early blocked sporulation mutants of Bacillus subtilis. Genetic study of polymyxin resistant partial revertants.
    Mol Gen Genet. 1971;112(3):243-54 PMID: 4331231
  12. Characterization of the spo0A locus and its deduced product.
    Proc Natl Acad Sci U S A. 1985 May;82(9):2647-51 PMID: 3157992
  13. Nucleotide sequence and transcription of a bacteriophage 29 early promoter.
    J Biol Chem. 1985 May 25;260(10):5950-5 PMID: 3997806
  14. Deduced product of the stage 0 sporulation gene spo0F shares homology with the Spo0A, OmpR, and SfrA proteins.
    Proc Natl Acad Sci U S A. 1985 Nov;82(21):7260-4 PMID: 2997779
  15. The complete sequence of the Bacillus phage phi 29 right early region.
    Gene. 1985;40(2-3):301-9 PMID: 3007295
  16. Two-component regulatory systems responsive to environmental stimuli share strongly conserved domains with the nitrogen assimilation regulatory genes ntrB and ntrC.
    Proc Natl Acad Sci U S A. 1986 Oct;83(20):7850-4 PMID: 3020561
  17. The effect of spo0 mutations on the expression of spo0A- and spo0F-lacZ fusions.
    Mol Gen Genet. 1986 Oct;205(1):28-33 PMID: 3099127
  18. Genetics of endospore formation in Bacillus subtilis.
    Annu Rev Genet. 1986;20:625-69 PMID: 3101583
  19. Regulation of spo0H, an early sporulation gene in bacilli.
    J Bacteriol. 1987 Mar;169(3):1182-91 PMID: 3102457
  20. Role of AbrB in Spo0A- and Spo0B-dependent utilization of a sporulation promoter in Bacillus subtilis.
    J Bacteriol. 1987 May;169(5):2223-30 PMID: 2437099
  21. Conserved domains in bacterial regulatory proteins that respond to environmental stimuli.
    Cell. 1987 Jun 5;49(5):579-81 PMID: 3555840
  22. Purification and characterization of the spoOA protein of Bacillus subtilis from an overproducing strain of Escherichia coli.
    Eur J Biochem. 1987 Sep 1;167(2):233-8 PMID: 3113950
  23. Bacillus sporulation gene spo0H codes for sigma 30 (sigma H).
    J Bacteriol. 1988 Mar;170(3):1054-62 PMID: 3277943
  24. Isolation and sequence of the spo0E gene: its role in initiation of sporulation in Bacillus subtilis.
    Mol Microbiol. 1987 Jul;1(1):125-32 PMID: 2838724
  25. Transmitter and receiver modules in bacterial signaling proteins.
    Proc Natl Acad Sci U S A. 1988 Jul;85(14):4981-5 PMID: 3293046
  26. Complete sequence and transcriptional analysis of the spo0F region of the Bacillus subtilis chromosome.
    J Bacteriol. 1988 Sep;170(9):4194-208 PMID: 2457578
  27. Structure of the gene for the transition state regulator, abrB: regulator synthesis is controlled by the spo0A sporulation gene in Bacillus subtilis.
    Mol Microbiol. 1988 Nov;2(6):689-99 PMID: 3145384
  28. Characterization of the gene for a protein kinase which phosphorylates the sporulation-regulatory proteins Spo0A and Spo0F of Bacillus subtilis.
    J Bacteriol. 1989 Nov;171(11):6187-96 PMID: 2509430
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.87.5.1801
Published
1990-03-00
Pages
1801-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC53571
Subset
IM
Grants
NIGMS NIH HHS · GM19416 · United States
NIGMS NIH HHS · GM39442 · United States
External Links
PubMed source DOI: 10.1073/pnas.87.5.1801 PMC Full Text View journal details
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