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PMID: 21073240 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Towards monitoring real-time cellular response using an integrated microfluidics-matrix assisted laser desorption ionisation/nanoelectrospray ionisation-ion mobility-mass spectrometry platform.

IET systems biology ·Vol. 4 ·No. 6 ·2010-11-00 ·Pages 416-27

Enders JR, Marasco CC, Kole A, Nguyen B, Sevugarajan S, Seale KT, Wikswo JP, McLean JA

Abstract

The combination of microfluidic cell trapping devices with ion mobility-mass spectrometry offers the potential for elucidating in real time the dynamic responses of small populations of cells to paracrine signals, changes in metabolite levels and delivery of drugs and toxins. Preliminary experiments examining peptides in methanol and recording the interactions of yeast and Jurkat cells with their superfusate have identified instrumental set-up and control parameters and online desalting procedures. Numerous initial experiments demonstrate and validate this new instrumental platform. Future outlooks and potential applications are addressed, specifically how this instrumentation may be used for fully automated systems biology studies of the significantly interdependent, dynamic internal workings of cellular metabolic and signalling pathways.

MeSH Terms
Cell Biology/instrumentation Cell Physiological Phenomena Cell Separation/instrumentation Cells/chemistry,cytology Humans Jurkat Cells Microfluidic Analytical Techniques/methods Saccharomyces cerevisiae Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods Systems Biology/methods
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Enders J R
Vanderbilt University, Department of Chemistry, Nashville, TN, USA.
Marasco C C
Kole A
Nguyen B
Sevugarajan S
Seale K T
Wikswo J P
McLean J A
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Article Info
Journal
IET systems biology
Abbr.
IET Syst Biol
ISSN
1751-8849
DOI
10.1049/iet-syb.2010.0012
Published
2010-11-00
Pages
416-27
Language
English
Region
England
NLM ID
101301198
PMCID
PMC4254925
Subset
IM
Grants
NIDA NIH HHS · RC2 DA028981 · United States
NIDA NIH HHS · RC2DA028981 · United States
External Links
PubMed source DOI: 10.1049/iet-syb.2010.0012 PMC Full Text View journal details
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