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PMID: 2110139 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Relationship between DNA cycle and growth rate in Synechococcus sp. strain PCC 6301.

Journal of bacteriology ·Vol. 172 ·No. 5 ·1990-05-00 ·Pages 2313-9

Binder BJ, Chisholm SW

Abstract

Flow cytometry was used to examine cell cycle regulation in Synechococcus sp. strain PCC 6301 under a variety of growth conditions. The DNA frequency distributions of exponentially growing and dark-blocked populations confirmed that this cyanobacterium contains multiple chromosome copies even at very slow growth rates. Furthermore, the presence of major peaks corresponding to other than 2" chromosome copies strongly suggests that DNA replication is initiated asynchronously. Although this suggestion is at odds with the standard formulation of the procaryotic cell cycle model, it is similar to recent observations of asynchrony in Escherichia coli replication mutants.

MeSH Terms
Cell Cycle Cyanobacteria/cytology,growth & development DNA Replication Darkness Flow Cytometry Kinetics Light
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Binder B J
Ralph M. Parsons Laboratory, Massachusetts Institute of Technology, Cambridge 02139.
Chisholm S W
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.172.5.2313-2319.1990
Published
1990-05-00
Pages
2313-9
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC208864
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.172.5.2313-2319.1990 PMC Full Text View journal details
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