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PMID: 2110147 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Characterization of PBSX, a defective prophage of Bacillus subtilis.

Journal of bacteriology ·Vol. 172 ·No. 5 ·1990-05-00 ·Pages 2667-74

Wood HE, Dawson MT, Devine KM, McConnell DJ

Abstract

PBSX, a defective Bacillus subtilis prophage, maps to the metA-metC region of the chromosome. DNA (33 kilobases) from this region of the chromosome was cloned and analyzed by insertional mutagenesis with the integrating plasmid pWD3. This plasmid had a promoterless alpha-amylase gene (amyL) that provided information on the direction and level of transcription at the site of integration. Transcription under the control of the PBSX repressor proceeded in the direction metA to metC over a distance of at least 18 kilobases. Electrophoretic analysis of proteins produced by different integrant strains upon PBSX induction and by fragments subcloned in Escherichia coli allowed the identification of early and late regions of the prophage. A set of contiguous fragments directing mutagenic integration suggested that the minimum size of an operon that encodes phage structural proteins is 19 kilobases. The adaptation of PBSX transcriptional and replicational functions to a chromosomally based, thermoinducible expression system is discussed.

MeSH Terms
Bacillus subtilis/genetics Bacteriophages/genetics Chromosome Mapping Chromosomes, Bacterial Cloning, Molecular Defective Viruses/genetics Escherichia coli/genetics Genes Mutation Plasmids Promoter Regions, Genetic Restriction Mapping Transduction, Genetic Transformation, Bacterial alpha-Amylases/genetics
Chemicals
alpha-Amylases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Wood H E
Department of Genetics, Trinity College, Dublin, Ireland.
Dawson M T
Devine K M
McConnell D J
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.172.5.2667-2674.1990
Published
1990-05-00
Pages
2667-74
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC208911
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.172.5.2667-2674.1990 PMC Full Text View journal details
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