Expression and role of p75 interleukin 2 receptor on human monocytes.

The Journal of experimental medicine ·Vol. 171 ·No. 5 ·1990-05-01 ·Pages 1821-6

Espinoza-Delgado I, Ortaldo JR, Winkler-Pickett R, Sugamura K, Varesio L, Longo DL

Abstract

We investigated the expression of IL-2R subunits in human monocytes using the TU27 mAb, which recognizes the p75 chain, and anti-Tac mAb, which recognizes the p55 moiety of the IL-2R. We found that p75 but not p55 is constitutively expressed in more than 90% of fresh human monocytes. Antibody to p75, but not to p55, inhibited the activation of monocytes to a cytotoxic stage induced by IL-2 but did not block IFN-gamma-induced cytotoxicity. Our data demonstrate that the p75 chain is expressed on human monocytes and is involved in the activation of monocytes by IL-2.

MeSH Terms

Antibodies, Monoclonal Cell Line Cytotoxicity, Immunologic Flow Cytometry Humans In Vitro Techniques Interferon-gamma/pharmacology Interleukin-2/pharmacology Lymphocyte Activation Lymphocytes/drug effects,immunology Macromolecular Substances Molecular Weight Monocytes/immunology Receptors, Interleukin-2/biosynthesis,isolation & purification

Chemicals

Antibodies, Monoclonal Interleukin-2 Macromolecular Substances Receptors, Interleukin-2 Interferon-gamma

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Espinoza-Delgado I

Biological Response Modifiers Program, National Cancer Institute, Frederick, Maryland 21701.

Ortaldo J R

Winkler-Pickett R

Sugamura K

Varesio L

Longo D L

References (10)

10 references, click to expand

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Article Info

Journal

The Journal of experimental medicine

Abbr.

J Exp Med

ISSN

0022-1007

DOI

10.1084/jem.171.5.1821

Published

1990-05-01

Pages

1821-6

Language

English

Region

United States

NLM ID

2985109R

PMCID

PMC2187901

Subset

External Links

PubMed source DOI: 10.1084/jem.171.5.1821 PMC Full Text View journal details

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Expression and role of p75 interleukin 2 receptor on human monocytes.

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