Home LiteratureArticle Details
PMID: 2111264 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The effects of chromosome rearrangements on the expression of heterochromatic genes in chromosome 2L of Drosophila melanogaster.

Genetics ·Vol. 125 ·No. 1 ·1990-05-00 ·Pages 141-54

Wakimoto BT, Hearn MG

Abstract

The light (lt) gene of Drosophila melanogaster is located at the base of the left arm of chromosome 2, within or very near centromeric heterochromatin (2Lh). Chromosome rearrangements that move the lt+ gene from its normal proximal position and place the gene in distal euchromatin result in mosaic or variegated expression of the gene. The cytogenetic and genetic properties of 17 lt-variegated rearrangements are described in this report. We show that five of the heterochromatic genes adjacent to lt are subject to inactivation by these rearrangements and that the euchromatic loci in proximal 2L are not detectably affected. The properties of the rearrangements suggest that proximity to heterochromatin is an important regulatory requirement for at least six 2Lh genes. We discuss how the properties of the position effects on heterochromatic genes relate to other proximity-dependent phenomena such as transvection.

MeSH Terms
Animals Chromosome Banding Chromosomes/metabolism Drosophila melanogaster/genetics Female Gene Expression Regulation Genes Genetic Complementation Test Heterochromatin Male Phenotype Pigmentation/genetics Translocation, Genetic Y Chromosome
Chemicals
Heterochromatin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Wakimoto B T
Department of Zoology NJ-15, University of Washington, Seattle 98195.
Hearn M G
References (36)
36 references, click to expand
  1. Modulation of tk expression in mouse pericentromeric heterochromatin.
    Mol Cell Biol. 1986 Dec;6(12):4440-9 PMID: 2432401
  2. V-Type Position Effects of a Gene in Drosophila Virilis Normally Located in Heterochromatin.
    Genetics. 1953 Jul;38(4):328-44 PMID: 17247441
  3. The Relation of a Dominant Eye Color in Drosophila Melanogaster to the Associated Chromosome Rearrangement.
    Genetics. 1934 Jul;19(4):344-64 PMID: 17246728
  4. Trans-inactivation of the Drosophila brown gene: evidence for transcriptional repression and somatic pairing dependence.
    Proc Natl Acad Sci U S A. 1989 Sep;86(17):6704-8 PMID: 2505257
  5. Reproducible compartmentalization of individual chromosome domains in human CNS cells revealed by in situ hybridization and three-dimensional reconstruction.
    Chromosoma. 1988;96(6):397-410 PMID: 3219911
  6. The genetics of dopa decarboxylase in Drosophila melanogaster. I. Isolation and characterization of deficiencies that delete the dopa-decarboxylase-dosage-sensitive region and the alpha-methyl-dopa-hypersensitive locus.
    Genetics. 1976 Oct;84(2):267-85 PMID: 826447
  7. Characterization and functional expression in mammalian cells of genomic and cDNA clones encoding a Drosophila muscarinic acetylcholine receptor.
    Proc Natl Acad Sci U S A. 1989 Nov;86(22):9039-43 PMID: 2510174
  8. X-Ray Induced Chromosomal Alterations in Drosophila Melanogaster.
    Genetics. 1938 Nov;23(6):610-30 PMID: 17246905
  9. Identification of a nonhistone chromosomal protein associated with heterochromatin in Drosophila melanogaster and its gene.
    Mol Cell Biol. 1986 Nov;6(11):3862-72 PMID: 3099166
  10. A simple method of reducing the fading of immunofluorescence during microscopy.
    J Immunol Methods. 1981;43(3):349-50 PMID: 7019347
  11. The role of X-chromosome inactivation during spermatogenesis (Drosophila-allocycly-chromosome evolution-male sterility-dosage compensation).
    Proc Natl Acad Sci U S A. 1972 Jan;69(1):182-6 PMID: 4621547
  12. The dichotornous action of Y chromosomes on the expression of position-effect variegation.
    Genetics. 1962 Oct;47:1399-407 PMID: 13969181
  13. Analysis of the functional significance of linkage group conservation in Drosophila.
    Genetics. 1987 Oct;117(2):233-44 PMID: 17246402
  14. Non-random position of the A-T rich DNA sequences in early embryos of Drosophila virilis.
    Chromosoma. 1981;83(4):555-61 PMID: 7273959
  15. Arrangement of chromatin in the nucleus.
    Hum Genet. 1980 Feb;53(2):131-43 PMID: 6987157
  16. V-Type Position Effects at the Light Locus in Drosophila Melanogaster.
    Genetics. 1958 May;43(3):395-403 PMID: 17247765
  17. Screening for x-ray-induced crossover suppressors in Drosophila melanogaster: prevalence and effectiveness of translocations.
    Genetics. 1970 Jul;65(3):429-48 PMID: 5519644
  18. On the components of segregation distortion in Drosophila melanogaster. III. Nature of enhancer of SD.
    Genetics. 1984 Jul;107(3):423-34 PMID: 6428976
  19. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.
    Anal Biochem. 1983 Jul 1;132(1):6-13 PMID: 6312838
  20. A Reconsideration of the Mechanism of Position Effect.
    Proc Natl Acad Sci U S A. 1944 Aug 15;30(8):183-97 PMID: 16588642
  21. Studies on the Position Effect at the Cubitus Interruptus Locus of Drosophila Melanogaster.
    Genetics. 1955 May;40(3):343-73 PMID: 17247557
  22. A structural basis for variegating position effects.
    Cell. 1984 Jul;37(3):869-78 PMID: 6086148
  23. Studies of normal and position-affected expression of rosy region genes in Drosophila melanogaster.
    Genetics. 1986 Nov;114(3):819-40 PMID: 3098623
  24. The organization and expression of the light gene, a heterochromatic gene of Drosophila melanogaster.
    Genetics. 1990 May;125(1):129-40 PMID: 2111263
  25. Dosage-dependent modifiers of position effect variegation in Drosophila and a mass action model that explains their effect.
    Genetics. 1988 Sep;120(1):181-98 PMID: 3146523
  26. Three-dimensional organization of Drosophila melanogaster interphase nuclei. II. Chromosome spatial organization and gene regulation.
    J Cell Biol. 1987 Jun;104(6):1471-83 PMID: 3108265
  27. The effect of chromosomal position on the expression of the Drosophila xanthine dehydrogenase gene.
    Cell. 1983 Aug;34(1):47-57 PMID: 6309411
  28. Genetic analysis of the proximal region of chromosome 2 of Drosophila melanogaster. I. Detachment products of compound autosomes.
    Genetics. 1975 Dec;81(4):705-21 PMID: 814038
  29. Determinants of heat shock-induced chromosome puffing.
    Cell. 1985 Apr;40(4):805-17 PMID: 3986904
  30. Differences in synaptic affinity of chromosome arms of Drosophila melanogaster revealed by differential sensitivity to translocation heterozygosity.
    Genetics. 1972 Jul;71(3):401-15 PMID: 4624919
  31. On the components of segregation distortion in Drosophila melanogaster. IV. Construction and analysis of free duplications for the Responder locus.
    Genetics. 1989 Apr;121(4):739-50 PMID: 2498160
  32. Further Characterization of Genetic Elements Associated with the Segregation Distorter Phenomenon in DROSOPHILA MELANOGASTER.
    Genetics. 1985 Aug;110(4):671-88 PMID: 17246295
  33. Variegation in Drosophila and the Inert Chromosome Regions.
    Proc Natl Acad Sci U S A. 1936 Jan;22(1):27-33 PMID: 16588038
  34. The underlying bases of gene expression differences in stable transformants of the rosy locus in Drosophila melanogaster.
    Genetics. 1986 Jun;113(2):265-85 PMID: 3013723
  35. Heterochromatin markers: arrangement of obligatory heterochromatin, histone genes and multisite gene families in the interphase nucleus of D. melanogaster.
    Chromosoma. 1982;86(4):443-55 PMID: 6816531
  36. Isolation of dominant suppressor mutations for position-effect variegation in Drosophila melanogaster.
    Mol Gen Genet. 1981;182(3):516-9 PMID: 6795427
Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/125.1.141
Published
1990-05-00
Pages
141-54
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1203996
Subset
IM
Grants
NIGMS NIH HHS · GM07735 · United States
NIGMS NIH HHS · GM34977 · United States
External Links
PubMed source DOI: 10.1093/genetics/125.1.141 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2