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PMID: 2112549 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Molecular genetics of Drosophila alpha-actinin: mutant alleles disrupt Z disc integrity and muscle insertions.

The Journal of cell biology ·Vol. 110 ·No. 6 ·1990-06-00 ·Pages 1999-2011

Fyrberg E, Kelly M, Ball E, Fyrberg C, Reedy MC

Abstract

We have isolated a Drosophila melanogaster alpha-actinin gene and partially characterized several mutant alleles. The Drosophila protein sequence is very similar (68% identity) to those of chicken alpha-actinin isoforms, but less closely related (30% identity) to Dictyostelium alpha-actinin. The gene is within subdivision 2C of the X chromosome, coincident with 15 lethal (1)2Cb mutations. At least four alleles, l(1)2Cb1, l(1)2Cb2, l(1)2Cb4, and l(1)2Cb5 are interrupted by rearrangement breakpoints and must be null. In all four cases, hemizygous mutants complete embryogenesis and do not die until the second day of larval growth, signifying that either the role of alpha-actinin in nonmuscle cells is redundant or that a distinct and only distantly related gene encodes the non-muscle isoform. Allelic but less severely affected fliA mutants are apparently due to point mutations, and develop into adults having thoracic muscle abnormalities. EM of mutant muscles reveals that Z discs and myofibrillar attachments are disrupted, whereas epithelial "tendon" cells are less affected. We discuss these phenotypes in the light of presumed in vivo alpha-actinin functions.

MeSH Terms
Actinin/analysis,genetics Alleles Amino Acid Sequence Amino Acids/analysis Animals Cell Membrane/physiology,ultrastructure Chromosome Mapping Cytoskeleton/physiology,ultrastructure Drosophila melanogaster/genetics Exons Molecular Sequence Data Muscles/cytology,physiology,ultrastructure Mutation
Chemicals
Amino Acids Actinin
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Fyrberg E
Department of Biology, Johns Hopkins University, Baltimore, Maryland 21218.
Kelly M
Ball E
Fyrberg C
Reedy M C
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43 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.110.6.1999
Published
1990-06-00
Pages
1999-2011
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2116134
Subset
IM
Databases
GENBANK
X51753
External Links
PubMed source DOI: 10.1083/jcb.110.6.1999 PMC Full Text View journal details
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