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PMID: 21142151 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

A photocleavable rapamycin conjugate for spatiotemporal control of small GTPase activity.

Journal of the American Chemical Society ·Vol. 133 ·No. 1 ·2011-01-12 ·Pages 12-4

Umeda N, Ueno T, Pohlmeyer C, Nagano T, Inoue T

Abstract

We developed a novel method to spatiotemporally control the activity of signaling molecules. A newly synthesized photocaged rapamycin derivative induced rapid dimerization of FKBP (FK-506 binding protein) and FRB (FKBP-rapamycin binding protein) upon UV irradiation. With this system and the spatially confined UV irradiation, we achieved subcellularly localized activation of Rac, a member of small GTPases. Our technique offers a powerful approach to studies of dynamic intracellular signaling events.

MeSH Terms
Animals HeLa Cells Humans Mice Models, Molecular NIH 3T3 Cells Photochemical Processes Protein Multimerization/drug effects Protein Structure, Quaternary Sirolimus/chemistry,pharmacology Tacrolimus Binding Proteins/chemistry,metabolism Time Factors rac GTP-Binding Proteins/metabolism
Chemicals
rac GTP-Binding Proteins Tacrolimus Binding Proteins Sirolimus
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Umeda Nobuhiro
Department of Cell Biology, Center for Cell Dynamics, Johns Hopkins University, Baltimore, Maryland 21205, USA.
Ueno Tasuku
Pohlmeyer Christopher
Nagano Tetsuo
Inoue Takanari
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Article Info
Journal
Journal of the American Chemical Society
Abbr.
J Am Chem Soc
ISSN
1520-5126
DOI
10.1021/ja108258d
Published
2011-01-12
Epub
2010-00-13
Pages
12-4
Language
English
Region
United States
NLM ID
7503056
PMCID
PMC3850177
Subset
IM
Grants
NIGMS NIH HHS · GM092930 · United States
NIMH NIH HHS · U54 MH084691 · United States
NIDDK NIH HHS · DK090868 · United States
NIDDK NIH HHS · P30 DK090868 · United States
NIGMS NIH HHS · R01 GM092930 · United States
External Links
PubMed source DOI: 10.1021/ja108258d PMC Full Text View journal details
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