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PMID: 2116006 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

A preparation of Alzheimer paired helical filaments that displays distinct tau proteins by polyacrylamide gel electrophoresis.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 87 ·No. 15 ·1990-08-00 ·Pages 5827-31

Greenberg SG, Davies P

Abstract

Paired helical filaments (PHFs) are prominent components of Alzheimer disease (AD) neurofibrillary tangles (NFTs). Rather than isolating NFTs, we selected for PHF populations that can be extracted from AD brain homogenates. About 50% of PHF immunoreactivity can be obtained in 27,200 x g supernatants following homogenization in buffers containing 0.8 M NaCl. We further enriched for PHFs by taking advantage of their insolubility in the presence of zwitterionic detergents and 2-mercaptoethanol, removal of aggregates by filtration through 0.45-microns filters, and sucrose density centrifugation. PHF-enriched fractions contained two to five proteins of 57-68 kDa that displayed the same antigenic properties as PHFs. Since the 57- to 68-kDa PHF proteins are antigenically related to tau proteins, they are similar to the tau proteins previously observed in NFTs. However, further analysis revealed that PHF-associated tau can be distinguished from normal, soluble tau by PHF antibodies that do not recognize human adult tau and by one- and two-dimensional PAGE.

MeSH Terms
Actin Cytoskeleton/ultrastructure Adult Aged Aged, 80 and over Alzheimer Disease/pathology Cell Fractionation Centrifugation, Density Gradient Cerebral Cortex/pathology,ultrastructure Cytoskeleton/ultrastructure Electrophoresis, Polyacrylamide Gel Humans Microscopy, Electron Microtubule-Associated Proteins/analysis,isolation & purification Middle Aged Nerve Tissue Proteins/analysis Parietal Lobe/ultrastructure Reference Values Temporal Lobe/ultrastructure tau Proteins
Chemicals
Microtubule-Associated Proteins Nerve Tissue Proteins tau Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Greenberg S G
Department of Pathology, Albert Einstein College of Medicine, Bronx, NY 10461.
Davies P
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.87.15.5827
Published
1990-08-00
Pages
5827-31
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC54421
Subset
IM
Grants
NIMH NIH HHS · MH38623 · United States
External Links
PubMed source DOI: 10.1073/pnas.87.15.5827 PMC Full Text View journal details
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