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PMID: 21178402 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Review

Dynamics of the Rho-family small GTPases in actin regulation and motility.

Cell adhesion & migration ·Vol. 5 ·No. 2 ·2011-00-00 ·Pages 170-80

Spiering D, Hodgson L

Abstract

The p21 Rho-family of small GTPases are master regulators of actin cytoskeleton rearrangements. Their functions have been well characterized in terms of their effects toward various actin-modulating protein targets. However, more recent studies have shown that the dynamics of Rho GTPase activities are highly complex and tightly regulated in order to achieve their specific subcellular localization. Furthermore, these localized effects are highly dynamic, often spanning the time-scale of seconds, making the interpretation of traditional biochemical approaches inadequate to fully decipher these rapid mechanisms in vivo. Here, we provide an overview of Rho family GTPase biology, and introduce state-of-the-art approaches to study the dynamics of these important signaling proteins that ultimately coordinate the actin cytoskeleton rearrangements during cell migration.

MeSH Terms
Actins/metabolism Animals Biosensing Techniques/methods Cell Migration Assays Cell Movement Cell Polarity Cytoskeletal Proteins/genetics,metabolism Cytoskeleton/enzymology Fluorescence Resonance Energy Transfer Gene Expression Green Fluorescent Proteins/analysis Immunoprecipitation Isoenzymes/genetics,metabolism Mice Optical Phenomena Polymerization Signal Transduction/physiology Time Factors rho GTP-Binding Proteins/genetics,metabolism
Chemicals
Actins Cytoskeletal Proteins Isoenzymes actin-modulating proteins Green Fluorescent Proteins rho GTP-Binding Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Spiering Désirée
Department of Anatomy and Structural Biology, Albert Einstein College of Medicine of Yeshiva University, Bronx, NY, USA.
Hodgson Louis
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Article Info
Journal
Cell adhesion & migration
Abbr.
Cell Adh Migr
ISSN
1933-6926
DOI
10.4161/cam.5.2.14403
Published
2011-00-00
Epub
2011-00-01
Pages
170-80
Language
English
Region
United States
NLM ID
101469464
PMCID
PMC3084983
Subset
IM
Grants
NIGMS NIH HHS · R01 GM093121 · United States
NIGMS NIH HHS · R01 GM093121-02 · United States
NIGMS NIH HHS · GM093121 · United States
External Links
PubMed source DOI: 10.4161/cam.5.2.14403 PMC Full Text View journal details
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