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PMID: 21220313 Published · ppublish English Journal Article Research Support, N.I.H., Extramural

Arabidopsis decuple mutant reveals the importance of SnRK2 kinases in osmotic stress responses in vivo.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 108 ·No. 4 ·2011-01-25 ·Pages 1717-22

Fujii H, Verslues PE, Zhu JK

Abstract

Osmotic stress associated with drought or salinity is a major factor that limits plant productivity. Protein kinases in the SNF1-related protein kinase 2 (SnRK2) family are activated by osmotic stress, suggesting that the kinases are involved in osmotic stress signaling. However, due to functional redundancy, their contribution to osmotic stress responses remained unclear. In this report, we constructed an Arabidopsis line carrying mutations in all 10 members of the SnRK2 family. The decuple mutant snrk2.1/2/3/4/5/6/7/8/9/10 grew poorly under hyperosmotic stress conditions but was similar to the wild type in culture media in the absence of osmotic stress. The mutant was also defective in gene regulation and the accumulation of abscisic acid (ABA), proline, and inositol 1,4,5-trisphosphate under osmotic stress. In addition, analysis of mutants defective in the ABA-activated SnRK2s (snrk2.2/3/6) and mutants defective in the rest of the SnRK2s (snrk2.1/4/5/7/8/9/10) revealed that SnRK2s are a merging point of ABA-dependent and -independent pathways for osmotic stress responses. These results demonstrate critical functions of the SnRK2s in mediating osmotic stress signaling and tolerance.

MeSH Terms
Abscisic Acid/pharmacology Amino Acid Sequence Arabidopsis/genetics,growth & development,metabolism Arabidopsis Proteins/genetics,metabolism Enzyme Activation/drug effects Gene Expression Profiling Inositol 1,4,5-Trisphosphate/metabolism Molecular Sequence Data Mutation Oligonucleotide Array Sequence Analysis Osmotic Pressure/physiology Plant Growth Regulators/pharmacology Proline/metabolism Protein Kinases/genetics,metabolism Protein Serine-Threonine Kinases/genetics,metabolism Reverse Transcriptase Polymerase Chain Reaction Sequence Homology, Amino Acid Signal Transduction/physiology
Chemicals
Arabidopsis Proteins Plant Growth Regulators SnRK2 protein, Arabidopsis Abscisic Acid Inositol 1,4,5-Trisphosphate Proline Protein Kinases SnRK2.10 protein, Arabidopsis OST1 protein, Arabidopsis Protein Serine-Threonine Kinases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Fujii Hiroaki
Department of Botany and Plant Sciences, University of California, Riverside, CA 92521, USA.
Verslues Paul E
Zhu Jian-Kang
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
1091-6490
DOI
10.1073/pnas.1018367108
Published
2011-01-25
Epub
2011-00-10
Pages
1717-22
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC3029766
Subset
IM
Grants
NIGMS NIH HHS · R01 GM059138 · United States
NIGMS NIH HHS · R01GM059138 · United States
External Links
PubMed source DOI: 10.1073/pnas.1018367108 PMC Full Text View journal details
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